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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Cortey, Martí | |
dc.contributor.author | Díaz, Iván | |
dc.contributor.author | Vidal, Anna | |
dc.contributor.author | Martín Valls, Gerard | |
dc.contributor.author | Franzo, Giovanni | |
dc.contributor.author | Gómez de Nova, Pedro José | |
dc.contributor.author | Darwich, Laila | |
dc.contributor.author | Puente Fernández, Héctor | |
dc.contributor.author | Carvajal Urueña, Ana María | |
dc.contributor.author | Martín, Marga | |
dc.contributor.author | Mateu, Enric | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2019 | |
dc.date.accessioned | 2024-04-03T07:59:08Z | |
dc.date.available | 2024-04-03T07:59:08Z | |
dc.identifier.citation | Cortey, M., Díaz, I., Vidal, A., Martín-Valls, G., Franzo, G., Gómez De Nova, P. J., Darwich, L., Puente, H., Carvajal, A., Martín, M., & Mateu, E. (2019). High levels of unreported intraspecific diversity among RNA viruses in faeces of neonatal piglets with diarrhoea. BMC Veterinary Research, 15, Article e441. https://doi.org/10.1186/S12917-019-2204-2 | es_ES |
dc.identifier.other | https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-019-2204-2 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/19335 | |
dc.description | © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | es_ES |
dc.description.abstract | Background: Diarrhoea is a major cause of death in neonate pigs and most of the viruses that cause it are RNA viruses. Next Generation Sequencing (NGS) deeply characterize the genetic diversity among rapidly mutating virus populations at the interspecific as well as the intraspecific level. The diversity of RNA viruses present in faeces of neonatal piglets suffering from diarrhoea in 47 farms, plus 4 samples from non-diarrhoeic piglets has been evaluated by NGS. Samples were selected among the cases submitted to the Veterinary Diagnostic Laboratories of Infectious Diseases of the Universitat Autònoma de Barcelona (Barcelona, Spain) and Universidad de León (León, Spain). Results: The analyses identified the presence of 12 virus species corresponding to 8 genera of RNA viruses. Most samples were co-infected by several viruses. Kobuvirus and Rotavirus were more commonly reported, with Sapovirus, Astrovirus 3, 4 and 5, Enterovirus G, Porcine epidemic diarrhoea virus, Pasivirus and Posavirus being less frequently detected. Most sequences showed a low identity with the sequences deposited in GenBank, allowing us to propose several new VP4 and VP7 genotypes for Rotavirus B and Rotavirus C. Conclusions: Among the cases analysed, Rotaviruses were the main aetiological agents of diarrhoea in neonate pigs. Besides, in a small number of cases Kobuvirus and Sapovirus may also have an aetiological role. Even most animals were co-infected in early life, the association with enteric disease among the other examined viruses was unclear. The NGS method applied successfully characterized the RNA virome present in faeces and detected a high level of unreported intraspecific diversity. | es_ES |
dc.language | eng | es_ES |
dc.publisher | BMC | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | RNA virus | es_ES |
dc.subject.other | Piglets | es_ES |
dc.subject.other | Diarrhoea | es_ES |
dc.subject.other | Next-generation sequencing | es_ES |
dc.title | High levels of unreported intraspecific diversity among RNA viruses in faeces of neonatal piglets with diarrhoea | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1186/s12917-019-2204-2 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/RYC-2015-17154 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1746-6148 | |
dc.journal.title | BMC Veterinary Research | es_ES |
dc.volume.number | 15 | es_ES |
dc.page.initial | 441 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 3109.11 Virología | es_ES |
dc.subject.unesco | 3104.08 Porcinos | es_ES |
dc.description.project | This work was partially supported by the National Institute of Research and Agricultural and Food Technology (INIA, reference E-RTA2015–0003-C02–01 and E-RTA2015–0003-C02–02). Both projects partially fund laboratory analyses and reagents. A. Vidal was supported by a PIF grant from the Universitat Autònoma de Barcelona. M. Cortey was funded by Spanish Ministry of Economy and Competitiveness, program Ramón y Cajal (grant RyC-2015-17154). The role of the funding bodies not included the design of the study, the sample collection or the analysis, neither the interpretation of data, nor the writing of the manuscript. | es_ES |
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