dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Balseiro Morales, Ana María | |
dc.contributor.author | Altuzarra, Raúl | |
dc.contributor.author | Vidal, Enric | |
dc.contributor.author | Moll, Xavier | |
dc.contributor.author | Espada, Yvonne | |
dc.contributor.author | Sevilla, Iker A. | |
dc.contributor.author | Domingo, Mariano | |
dc.contributor.author | Garrido, Joseba M. | |
dc.contributor.author | Juste, Ramón A. | |
dc.contributor.author | Prieto, Miguel | |
dc.contributor.author | Pérez de Val, Bernat | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2017 | |
dc.date.accessioned | 2024-04-12T12:03:35Z | |
dc.date.available | 2024-04-12T12:03:35Z | |
dc.identifier.citation | Balseiro, A., Altuzarra, R., Vidal, E., Moll, X., Espada, Y., Sevilla, I. A., Domingo, M., Garrido, J. M., Juste, R. A., Prieto, M., & Pérez de Val, B. (2017). Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep. PLoS ONE, 12(7), Article e0180546. https://doi.org/10.1371/JOURNAL.PONE.0180546 | es_ES |
dc.identifier.other | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180546 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/19718 | |
dc.description.abstract | [EN]Background/Aims: Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study were to develop an experimental TB infection model in sheep with a Mycobacterium caprae field strain isolated from a tuberculous diseased ewe, and to use this to evaluate the safety and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. Methods: Eighteen 2 month-old lambs were experimentally challenged with M. caprae by the endotracheal route (1.5 × 103 CFU). They were separated per treatment group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n = 6). Clinical, immunological, pathological and bacteriological parameters of infection were measured. Results: All lambs were successfully infected and developed gross TB lesions in the respiratory system. The BCG vaccine conferred considerable protection against experimental TB in lambs, as measured by a reduction of the gross lesion volumes and bacterial load. However, HIMB vaccinated animals did not show protection. Conclusions: This study proposes a reliable new experimental model for a better understanding of tuberculosis in sheep. BCG vaccination offers an effective prospect for controlling the disease. Moreover alternative doses and/or routes of administration should be considered to evaluate the efficacy of the HIMB vaccine candidate. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | BCG vaccine | es_ES |
dc.subject.other | Mycobacterium bovis | es_ES |
dc.subject.other | Vacunas (Veterinaria) | es_ES |
dc.subject.other | Ganado ovino | es_ES |
dc.title | Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0180546 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2014-00002-C02-01/ES/Estrategias de control de la tuberculosis bovina en reservorios silvestres (tejón y jabalí) y domésticos (ovino) | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1932-6203 | |
dc.journal.title | PLOS ONE | es_ES |
dc.volume.number | 12 | es_ES |
dc.issue.number | 7 | es_ES |
dc.page.initial | e0180546 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 3104.07 Ovinos | es_ES |
dc.subject.unesco | 2412.10 Vacunas | es_ES |
dc.description.project | We are grateful to Albert Canturri (UAB), Maite Martín, Zoraida Cervera and the staff of the BSL-3 Unit of IRTA-CReSA for their technical support. We also thank Kevin P. Dalton for critically reviewing the manuscript. IRTA is supported by CERCA Programme / Generalitat de Catalunya. | es_ES |