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Título
New insights about functional and cross-reactive properties of antibodies generated against recombinant TbpBs of Haemophilus parasuis
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Scientific Reports
Número de la revista
1
Datos de la obra
Barasuol, B. M., Guizzo, J. A., Fegan, J. E., Martínez-Martínez, S., Rodríguez-Ferri, E. F., Gutiérrez-Martín, C. B., Kreutz, L. C., Schryvers, A. B., & Frandoloso, R. (2017). New insights about functional and cross-reactive properties of antibodies generated against recombinant TbpBs of Haemophilus parasuis. Scientific Reports, 7(1), Article e10377. https://doi.org/10.1038/S41598-017-10627-0
Editor
Nature Research
Fecha
2017
Abstract
[EN] Vaccines have become fundamental in the control and elimination of Glässer Disease, a systemic disease of pigs caused by Haemophilus parasuis. The classic vaccines available for prevention of this infection were developed without a robust knowledge about host immunological mechanisms. In this study, we demonstrated the presence of cross-reactive epitopes on both the N-lobe and C-lobe of variants of transferrin binding protein B (TbpBs) expressed on the surface of 6 virulent serovars of H. parasuis. Antibodies against TbpB-derived antigens were capable of increasing the phagocytic capacity of neutrophils and were also capable of blocking porcine transferrin from binding to TbpB. Surprisingly, none of the pig or mice antisera from animals immunized with TbpB-derived antigens mixed with Montanide IMS 2215 VG PR adjuvant were able to activate the classical complement pathway (CCP). In contrast, antisera from mice immunized with TbpB-derived antigens adjuvanted with Freund's adjuvants or Montanide Gel 01 were able to activate the CCP and kill H. parasuis. Our results demonstrate that the type of adjuvant can modulate the functional response induced by TbpB-derived antigens. Based on these results, we propose that a properly formulated TbpB-based vaccine may elicit a functional protective antibody response with broad cross-reactivity against heterologous strains of H. parasuis.
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Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2011-23195/ES/PROTEINAS RECOMBINANTES DE HAEMOPHILUS PARASUIS CON AFINIDAD POR LA TRANSFERRINA PORCINA. VALORACION DE SU INMUNIDAD PROTECTORA EN LA ENFERMEDAD DE GLASSER//
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