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Título
Viability and Virulence of Listeria monocytogenes in Poultry
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Microorganisms
Número de la revista
9
Datos de la obra
Panera Martínez, S., Capita, R., García Fernández, C., & Alonso Calleja, C. (2023). Viability and Virulence of Listeria monocytogenes in Poultry. Microorganisms, 11(9), Article e2232. https://doi.org/10.3390/MICROORGANISMS11092232
Editor
MDPI
Fecha
2023
Abstract
[EN] The prevalence of Listeria monocytogenes in 30 samples of poultry was determined using culture-dependent (isolation on OCLA and confirmation by conventional polymerase chain reaction -PCR-, OCLA&PCR) and culture-independent (real-time polymerase chain reaction, q-PCR) methods. L. monocytogenes was detected in 15 samples (50.0%) by OCLA&PCR and in 20 (66.7%) by q-PCR. The concentrations (log10 cfu/g) of L. monocytogenes (q-PCR) ranged from 2.40 to 5.22 (total cells) and from <2.15 to 3.93 (viable cells). The two methods, q-PCR using a viability marker (v-PCR) and OCLA&PCR (gold standard), were compared for their capacity to detect viable cells of L. monocytogenes, with the potential to cause human disease. The values for sensitivity, specificity and efficiency of the v-PCR were 100%, 66.7% and 83.3%, respectively. The agreement between the two methods (kappa coefficient) was 0.67. The presence of nine virulence genes (hlyA, actA, inlB, inlA, inlC, inlJ, prfA, plcA and iap) was studied in 45 L. monocytogenes isolates (three from each positive sample) using PCR. All the strains harbored between six and nine virulence genes. Fifteen isolates (33.3% of the total) did not show the potential to form biofilm on a polystyrene surface, as determined by a crystal violet assay. The remaining strains were classified as weak (23 isolates, 51.1% of the total), moderate (one isolate, 2.2%) or strong (six isolates, 13.3%) biofilm producers. The strains were tested for susceptibility to a panel of 15 antibiotics. An average of 5.11 ± 1.30 resistances per isolate was observed. When the values for resistance and for reduced susceptibility were taken jointly, this figure rose to 6.91 ± 1.59. There was a prevalence of resistance or reduced susceptibility of more than 50.0% for oxacillin, cefoxitin, cefotaxime, cefepime ciprofloxacin, enrofloxacin and nitrofurantoin. For the remaining antibiotics tested, the corresponding values ranged from 0.0% for chloramphenicol to 48.9% for rifampicin. The high prevalence and level of L. monocytogenes with numerous virulence factors in poultry underline how crucial it is to follow correct hygiene procedures during the processing of this foodstuff in order to reduce the risk of human listeriosis.
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- info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018- 098267-R-C33/ES/ALTERNATIVAS AL USO DE DESINFECTANTES EN LA INDUSTRIA ALIMENTARIA DIRIGIDAS A REDUCIR LA SUPERVIVENCIA DE LISTERIA MONOCYTOGENES Y SALMONELLA ENTERICA SOBRE LAS SUPERFICIES
- info:eu-repo/grantAgreement/AEI/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia/PID2022-142329OB-C31/ES/CARACTERIZACION DE LA MICROBIOTA EN SUPERFICIES DE INDUSTRIAS ALIMENTARIAS: EFECTO DE NUEVOS BIOCIDAS SOBRE LA RESISTENCIA A ANTIBIOTICOS Y LOS BIOFILMS DE L. MONOCYTOGENES//
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