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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Silva, Vanessa | |
dc.contributor.author | Correira, Elisete | |
dc.contributor.author | Pereira, José Eduardo | |
dc.contributor.author | González Machado, Camino | |
dc.contributor.author | Capita González, Rosa María | |
dc.contributor.author | Alonso Calleja, Carlos | |
dc.contributor.author | Igrejas, Gilberto | |
dc.contributor.author | Poeta, Patrícia | |
dc.contributor.other | Nutricion y Bromatologia | es_ES |
dc.date | 2022 | |
dc.date.accessioned | 2024-05-02T09:02:15Z | |
dc.date.available | 2024-05-02T09:02:15Z | |
dc.identifier.citation | Silva, V., Correia, E., Pereira, J. E., González Machado, C., Capita, R., Alonso Calleja, C., Igrejas, G., & Poeta, P. (2022). Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species. Pathogens, 11(6), Article e689. https://doi.org/10.3390/PATHOGENS11060689 | es_ES |
dc.identifier.other | https://www.mdpi.com/2076-0817/11/6/689 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/20244 | |
dc.description | Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.description.abstract | [EN] The ability of biofilm formation seems to play an important role in the virulence of staphylococci. However, studies reporting biofilm formation of coagulase-negative staphylococci isolated from animals are still very scarce. Thus, we aimed to evaluate the biofilm-forming capacity of CoNS and S. pseudintermedius isolated from several animal species and to investigate the effect of conventional antimicrobials on biofilm reduction. A total of 35 S. pseudintermedius and 192 CoNS were included. Biofilm formation was accessed by the microtiter plate assay and the biofilms were stained by crystal violet. Association between biofilm formation and staphylococci species and antimicrobial resistance was also performed. Biofilm susceptibility testing was performed with tetracycline and amikacin at the minimum inhibitory concentration (MIC) and 10 × MIC. The metabolic activity of the biofilm cells after antimicrobial treatment was accessed by the XTT assay. All isolates formed biofilm, with S. urealyticus producing the most biofilm biomass and S. pseudintermedius producing the least biomass. There was a positive association between biofilm formation and multidrug resistance as well as resistance to individual antimicrobials. Neither tetracycline nor amikacin were able to eradicate the biofilm, not even at the highest concentration used. This study provides new insights into biofilm formation and the effects of antimicrobials on CoNS species. | es_ES |
dc.language | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Tecnología de los alimentos | es_ES |
dc.subject.other | Biofilm | es_ES |
dc.subject.other | Staphylococci | es_ES |
dc.subject.other | Coagulase-negative staphylococci (CoNS) | es_ES |
dc.title | Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.3390/PATHOGENS11060689 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-098267-R-C33/ES/ALTERNATIVAS AL USO DE DESINFECTANTES EN LA INDUSTRIA ALIMENTARIA DIRIGIDAS A REDUCIR LA SUPERVIVENCIA DE LISTERIA MONOCYTOGENES Y SALMONELLA ENTERICA SOBRE LAS SUPERFICIES// | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 2076-0817 | |
dc.journal.title | Pathogens | es_ES |
dc.volume.number | 11 | es_ES |
dc.issue.number | 6 | es_ES |
dc.page.initial | 689 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2414.04 Bacteriología | es_ES |
dc.subject.unesco | 3309 Tecnología de Los Alimentos | es_ES |
dc.subject.unesco | 3109.05 Microbiología | es_ES |
dc.description.project | This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020) and by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). The Ministerio de Ciencia, Innovación y Universidades (Spain, grant number RTI2018-098267-R-C33) and the Junta de Castilla y León (Consejería de Educación, Spain, grant number LE018P20). Vanessa Silva is grateful to FCT (Fundacão para a Ciência e a Tecnologia) for financial support through the PhD grant SFRH/BD/137947/2018. | es_ES |
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