Compartir
Título
Antibacterial Action Mechanisms of Honey: Physiological Effects of Avocado, Chestnut, and Polyfloral Honey upon Staphylococcus aureus and Escherichia coli
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Molecules
Número de la revista
5
Cita Bibliográfica
Combarros Fuertes, P., Estevinho, L. M., Teixeira Santos, R., Rodrigues, A. G., Pina Vaz, C., Fresno, J. M., & Eugenia Tornadijo. (2020). Antibacterial action mechanisms of honey: Physiological Effects of Avocado, Chestnut, and Polyfloral Honey upon Staphylococcus aureus and Escherichia coli. Molecules, 25(5). https://doi.org/10.3390/MOLECULES25051252
Editorial
MDPI
Fecha
2020
Resumen
[EN] Numerous studies have explored the antibacterial properties of different types of honey from all around the world. However, the data available describing how honey acts against bacteria are few. The aim of this study was to apply a flow cytometry (FC) protocol to examine and characterize the primary effects of three varieties of honey (avocado, chestnut and polyfloral) upon physiological status of Staphylococcus aureus and Escherichia coli cells to reveal their antibacterial action mechanisms. The effects of honey samples on membrane potential, membrane integrity, and metabolic activity were assessed using different fluorochromes, in a 180 min time course assay. Time-kill experiments were also carried out under similar conditions. Exposure of S. aureus and E. coli to the distinct honey samples resulted in physiological changes related to membrane polarization and membrane integrity. Moreover, honey induced a remarkable metabolic disruption as primary physiological effect upon S. aureus. The different honey samples induced quite similar effects on both bacteria. However, the depth of bacteria response throughout the treatment varied depending on the concentration tested and among honey varieties, probably due to compositional differences in the honey
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/ Junta de Castilla y León/ Proyectos de I+D+I (CCAA)/ GRS 551/A/10/ES/ LA MIEL COMO ALIMENTO FUNCIONAL Y SUS APLICACIONES EN LA PREVENCIÓN Y TRATAMIENTO DE LAS COMPLICACIONES DEL TRATAMIENTO ONCOLÓGICO//
URI
DOI
Versión del editor
Aparece en las colecciones
- Artículos [5268]
Ficheros en el ítem
Tamaño:
931.9
xmlui.dri2xhtml.METS-1.0.size-kilobytes
Formato:
Adobe PDF