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Título
Formulation of a Highly Effective Inoculant for Common Bean Based on an Autochthonous Elite Strain of Rhizobium leguminosarum bv. phaseoli, and Genomic-Based Insights Into Its Agronomic Performance
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Título de la revista
Frontiers in Microbiology
Datos de la obra
Pastor-Bueis, R., Sánchez-Cañizares, C., James, E. K., & González-Andrés, F. (2019). Formulation of a Highly Effective Inoculant for Common Bean Based on an Autochthonous Elite Strain of Rhizobium leguminosarum bv. phaseoli, and Genomic-Based Insights Into Its Agronomic Performance. Frontiers in Microbiology, 10. https://doi.org/10.3389/FMICB.2019.02724
Editor
Frontiers Media
Fecha
2019
Abstract
[EN] Common bean is a poor symbiotic N-fixer, with a low response to inoculation
owing to its promiscuous nodulation with competitive but inefficient resident rhizobia.
Consequently, farmers prefer to fertilize them rather than rely on their capacity for
Biological Nitrogen Fixation (BNF). However, when rhizobial inoculants are based on
autochthonous strains, they often have superior BNF performance in the field due
to their genetic adaptations to the local environment. Nevertheless, there is scant
information at the genomic level explaining their superiority or on how their genomes
may influence the inoculant performance. This information is especially important in
technologically advanced agri-systems like Europe, where environmental concerns
and increasingly stringent fertilizer regulations are encouraging a return to the use of
rhizobial inoculants, but based upon strains that have been thoroughly characterized in
terms of their symbiotic performance and their genetics. The aim of this study was to
design an inoculant formulation based on a superior autochthonous strain, Rhizobium
leguminosarum bv. phaseoli LCS0306, to assess its performance in the field, and to
determine the genomic features contributing to the high effectiveness of its symbiosis
with common bean. Plants inoculated with the autochthonous strain LCS0306 fixed
significantly more nitrogen than those with the allochthonous strains R. phaseoli ATCC
14482T and R. etli CFN42T, and had grain yield similar to the nitrogen-fertilized
controls. Inoculation with LCS0306 was particularly efficacious when formulated with
a carrier based upon a mixture of perlite and biochar. Whole genome comparisons
revealed no differences in the classical symbiotic genes of strain LCS0306 within the
symbiovar phaseoli. However, its symbiotic superior performance might be due to its genomic versatility, as it harbors a large assortment of genes contributing to fitness
and competitiveness. It is concluded that inoculation with elite rhizobia formulated with
perlite-biochar carriers might constitute a step-change in the sustainable cultivation of
common bean in Spanish soils.
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