dc.description.abstract | Background: The pathogenesis of Haemophilus parasuis depends on the bacterium’s ability to interact with
endothelial cells and invade adjacent tissues. In this study, we investigated the abilities of eight H. parasuis
reference strains belonging to serovars 1, 2, 4, 5, 7, 9, 10 and 13 to adhere to and invade porcine aortic endothelial
cells (AOC-45 cell line).
Results: The strains belonging to serovars 1, 2 and 5 were able to attach at high rates between 60 and 240 min of
incubation, and serovars 4, 7 and 13 had moderate attachment rates; however, the strains belonging to serovars 9
and 10 had low adherence at all time points. Strong adherence was observed by scanning electron microscopy for
the strains of serovars 5 and 4, which had high and moderate numbers, respectively, of H. parasuis cells attached to
AOC-45 cells after 240 min of incubation. The highest invasiveness was reached at 180 min by the serovar 4 strain,
followed by the serovar 5 strain at 240 min. The invasion results differed substantially depending on the strain.
Conclusion: The reference strains of H. parasuis serovars 1, 2, 4 and 5 exhibited high adhesion and invasion levels
to AOC-45 porcine aorta endothelial cells, and these findings could aid to better explain the pathogenesis of the
disease caused by these serovars. | es_ES |