RT info:eu-repo/semantics/article
T1 Decay of sperm obtained from epididymes of wild ruminants depending on postmortem time
A1 Martínez Pastor, Felipe
A1 Guerra, Camino
A1 Kaabi, Mohammed
A1 Díaz Reyes, Alexey
A1 Anel Rodríguez, Enrique
A1 Paz Cabello, Paulino de
A1 Anel Rodríguez, Luis
A2 Biologia Celular
K1 Veterinaria
K1 Red deer
K1 Roe deer
K1 Postmortem recovery
K1 Epididymal sperm
K1 Refrigeration
K1 Epididymal storage
AB We have carried out a study on the effect of postmortem time (PT) in some characteristics of epididymal sperm salvaged from hunted Iberian red deer and roe deer. Testis were collected, identified, refrigerated down to 5 °C, and sent to our laboratory by the wardens of the hunting reserves. This way, samples were delivered at different times postmortem. Sperm were extracted from the cauda epididymis by means of cuts. Analyzed parameters were: osmolality, pH, motility—both subjectively and with CASA, HOS test reactivity, acrosomal status and viability (assessed with propidium iodide). Osmolality and pH rose with prolonged postmortem time, possibly due to tissue decomposition. Most sperm quality parameters negatively correlated with PT. Besides, when comparing PT classes (groups of 24 h for red deer and 30 h for roe deer), we could appreciate that motility was more affected by PT than other quality variables. Progressive motility was especially impaired. We also classified the samples in high, medium and low quality for each PT group (considering progressive motility, intact acrosomes and reactivity to the HOS test), and it was clear that after 2 days the number of high quality samples was testimonial, and after several days, we almost found only low quality samples. In conclusion, epididymal sperm from Iberian red deer and roe deer undergo a decrease of quality with PT, but it could stay acceptable within many hours postmortem. There are implications for wildlife conservation programs, as epididymal sperm is a good source of germplasm. If valuable animals die and it is not possible to process their sperm immediately, it may still be possible to obtain viable spermatozoa many hours later.
PB Elsevier
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10335
UL http://hdl.handle.net/10612/10335
NO Theriogenology, 2005, vol. 63, n. 1
NO P. 24-40
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 16-may-2024