RT info:eu-repo/semantics/article
T1 Rainbow trout surviving infections of viral haemorrhagic septicemia virus (vhsv) show lasting antibodies to recombinant g protein fragments
A1 Gómez-Casado, E.
A1 Fregeneda Grandes, Juan Miguel
A1 Olesen, Niels Jørgen
A1 Lorenzen, Niels
A1 Estepa Pérez, Amparo
A1 Coll Morales, Julio
A1 Encinas, Paloma
A2 Sanidad Animal
K1 Inmunología
K1 Sanidad animal
K1 Veterinaria
K1 Zoología
K1 Truchas
K1 Inmunoglobulinas
K1 Serología
AB Rainbow trout antibodies (Abs) binding to recombinant fragments (frgs) derived from the protein G of theviral haemorrhagic septicemia virus (VHSV)-07.71 strain, could be detected by ELISA (frg-ELISA) insera from trout surviving laboratory-controlled infections. Abs were detected not only by using sera fromtrout infected with the homologous VHSV isolate but also with the VHSV-DK-201433 heterologousisolate, which had 13 amino acid changes. Sera from healthy trout and/or from trout surviving infectioushaematopoietic necrosis virus (IHNV) infection, were used to calculate cut-off absorbances todifferentiate negative from positive sera. Specific anti-VHSV Abs could then be detected by using any ofthe following frgs: frg11 (56-110), frg15 (65-250), frg16 (252-450) or G21-465. While high correlationswere found among the ELISA values obtained with the different frgs, no correlations between any frg-ELISA and complement-dependent 50 % plaque neutralization test (PNT) titres could be demonstrated.Between 4 to 10 weeks after VHSV-infection, more trout sera were detected as positives by usingheterologous frg-ELISA rather than homologous PNT. Furthermore, the percentage of positive seradetected by frg11-ELISA increased with time after infection to reach 100 %, while those detected bycomplement-dependent PNT decreased to 29.4 %, thus confirming that the lack of neutralising Abs doesnot mean the lack of any anti-VHSV Abs in survivor trout sera. Preliminary results with sera from fieldsamples suggest that further refinements of the frg-ELISA could allow detection of anti-VHSV trout Absin natural outbreaks caused by different heterologous VHSV isolates.The homologous frg-ELISA methodcould be useful to follow G immunization attempts during vaccine development and/or to bestunderstand the fish Ab response during VHSV infections. The viral frgs approach might also be used withother fish species and/or viruses.
PB Elsevier
YR 2019
FD 2019-04-23
LK http://hdl.handle.net/10612/10571
UL http://hdl.handle.net/10612/10571
NO Fish and Shellfish Immunology, 2011, vol. 30, n. 3
NO P. 929-935
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 01-jun-2024