RT info:eu-repo/semantics/article
T1 Effects on brown bear (Ursus arctos) spermatozoa freezability of different extender and dilution ratios used for pre-freezing centrifugation
A1 Nicolás, M.
A1 Álvarez García, Mercedes
A1 Gomes Alves, Susana Cláudia
A1 Mata Campuzano, María
A1 Barragán Santos, Santiago
A1 Martínez Pastor, Felipe
A1 Paz Cabello, Paulino de
A1 Anel Rodríguez, Luis
A2 Biologia Celular
K1 Veterinaria
K1 Brown bear
K1 Spermatozoa
K1 Centrifugation
K1 Extender
K1 Dilution
AB The objective of this study was to determine how the extender and dilution ratio used during centrifugation affect bear spermatozoa quality before and after freezing–thawing. Semen was collected from 15 brown bears by electroejaculation. In experiment 1, semen was divided into five aliquots and diluted using one of the following extenders: Tris-citric-glucose (TCG), Tris-citric-glucose-3% BSA, Tris-citric-glucose-1% egg yolk or CaninePro. In experiment 2, semen was divided into five aliquots and diluted 1:1, 1:4, 1:8 or 1:16 (semen:extender) with Tris-citric-glucose. In both experiments, one aliquot was left undiluted and it was used as a control. All the aliquots were centrifuged at 600×g for 6 min and frozen. Samples were analysed by post-thawing for motility (CASA) and, by flow cytometry, for viability (YO-PRO-1), acrosomal status (PNA-FITC/PI) and mitochondrial status (JC-1). CaninePro rendered the highest motility with respect to the undiluted control (total motility, 53.1% vs. 38.5%, P < 0.001), and CaninePro and TCG significantly increased the percentage of viable and acrosome-intact spermatozoa (43.2 and 43.4, respectively, vs. 39.4, P < 0.05). In experiment 2, dilution 1:4 yielded the highest value of total motility (78.8 vs. 67.2, P < 0.05) and proportion of spermatozoa with intact membrane and acrosome (64.5 vs. 54.4, P < 0.01). In general, diluting 1:4 or 1:8 brown bear semen prior to centrifugation improved the motility and acrosome status of the thawed spermatozoa.
PB Springer
YR 2019
FD 2019-05-16
LK http://hdl.handle.net/10612/10769
UL http://hdl.handle.net/10612/10769
NO European Journal of Wildlife Research, 2011, vol. 57, n. 2
NO P. 259–266
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 17-may-2024