RT info:eu-repo/semantics/article
T1 Unraveling the emergence and population diversity of Listeria monocytogenes in a newly built meat facility through whole genome sequencing
A1 Álvarez Molina, Adrián
A1 Cobo Díaz, José Francisco
A1 López Fernández, María Mercedes
A1 Prieto Maradona, Miguel
A1 Álvarez Ordóñez, Avelino
A1 Toro, María de
A2 Tecnologia de los Alimentos
K1 Genética
K1 Tecnología de los alimentos
K1 Listeria monocytogenes
K1 Whole genome sequencing
K1 Persistence
K1 Food processing environment
K1 Plasmids
AB The food processing environments of a newly opened meat processing facility were sampled in ten visits carriedout during its first 1.5 years of activity and analyzed for the presence of Listeria monocytogenes. A total of 18L. monocytogenes isolates were obtained from 229 samples, and their genomes were sequenced to performcomparative genomic analyses. An increase in the frequency of isolation of L. monocytogenes and in the diversityof sequence types (STs) detected was observed along time. Although the strains isolated belonged to six differentSTs (ST8, ST9, ST14, ST37, ST121 and ST155), ST9 was the most abundant (8 out of 18 strains). Low (0 and 2)single nucleotide polymorphism (SNP) distances were found between two pairs of ST9 strains isolated in bothcases 3 months apart from the same processing room (Lm-1267 and Lm-1705, with a 2 SNPs distance in the coregenome; Lm-1265 and Lm-1706, with a 0 SNPs distance), which suggests that these strains may be persistentL. monocytogenes strains in the food processing environment. Most strains showed an in silico attenuated viru­lence potential either through the truncation of InlA (in 67% of the isolates) or the absence of other virulencefactors involved in cell adhesion or invasion. Twelve of the eighteen L. monocytogenes isolates contained aplasmid, which ranged in size from 4 to 87 Kb and harbored stress survival, in addition to heavy metals andbiocides resistance determinants. Identical or highly similar plasmids were identified for various sets ofL. monocytogenes ST9 isolates, which suggests the clonal expansion and persistence of plasmid-containing ST9strains in the processing environments of the meat facility. Finally, the analysis of the L. monocytogenes genomesavailable in the NCBI database, and their associated metadata, evidenced that strains from ST9 are morefrequently reported in Europe, linked to foods, particularly to meat and pork products, and less representedamong clinical isolates than other L. monocytogenes STs. It also showed that the ST9 strains here isolated weremore closely related to the European isolates, which clustered together and separated from ST9 North Americanisolates.
SN 0168-1605
LK http://hdl.handle.net/10612/13412
UL http://hdl.handle.net/10612/13412
NO -
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 15-jun-2024