RT info:eu-repo/semantics/article
T1 Susceptibility and transcriptomic response to plasma-activated water of Listeria monocytogenes planktonic and sessile cells
A1 Fernández Gómez, Paula
A1 Cobo Díaz, José Francisco
A1 Sousa Oliveira, Marcia Patricia de
A1 González Raurich, María Montserrat
A1 Álvarez Ordóñez, Avelino
A1 Prieto Maradona, Miguel
A1 Walsh, James L.
A1 Sivertsvik, Morten
A1 Noriega Fernández, Estefanía
A1 López Fernández, María Mercedes
A2 Tecnologia de los Alimentos
K1 Tecnología de los alimentos
K1 Plasma-activated water
K1 Antimicrobial activity
K1 Listeria monocytogenes
K1 Transcriptomics
K1 Anti-Biofilm
AB [EN] Plasma-Activated Water (PAW) was generated from tap water using a surface dielectric barrier discharge at different discharge power (26 and 36 W) and activation time (5 and 30 min). The inactivation of a three-strain Listeria monocytogenes cocktail in planktonic and biofilm state was evaluated. PAW generated at 36 W-30 min showed the lowest pH and the highest hydrogen peroxide, nitrates, nitrites contents and effectiveness against cells on planktonic state, resulting in 4.6 log reductions after a 15-min treatment. Although the antimicrobial activity in biofilms formed on stainless steel and on polystyrene was lower, increasing the exposure time to 30 min allowed an inactivation >4.5 log cycles. The mechanisms of action of PAW were investigated using chemical solutions that mimic its physico-chemical characteristics and also RNA-seq analysis. The main transcriptomic changes affected carbon metabolism, virulence and general stress response genes, with several overexpressed genes belonging to the cobalamin-dependent gene cluster.
PB Elsevier
SN 0740-0020
LK http://hdl.handle.net/10612/15844
UL http://hdl.handle.net/10612/15844
NO Fernández-Gómez, P., Cobo-Díaz, J. F., Oliveira, M., González-Raurich, M., Alvarez-Ordóñez, A., Prieto, M., Walsh, J. L., Sivertsvik, M., Noriega-Fernández, E., & López, M. (2023). Susceptibility and transcriptomic response to plasma-activated water of Listeria monocytogenes planktonic and sessile cells. Food Microbiology, 113(104252), 104252. https://doi.org/10.1016/j.fm.2023.104252
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 17-may-2024