RT info:eu-repo/semantics/article
T1 miR-29a is downregulated in progenies derived from chronically stressed males
A1 Fernández Riesco, Marta
A1 García Valcarce, David
A1 Sellés Egea, Alba
A1 Esteve Codina, Anna
A1 Herráez Ortega, María Paz
A1 Robles Rodríguez, Vanesa
A2 Bioquimica y Biologia Molecular
K1 Biología
K1 Chronic stress
K1 Offspring
K1 RNA-seq
K1 Small-RNAs
K1 Behavior
K1 Hatching rates
K1 Survival
K1 Malformations
K1 2415 Biología Molecular
AB [EN] Recent research has provided compelling evidence demonstrating that paternal exposureto different stressors can influence their offspring’s phenotypes. We hypothesized that paternal stresscan negatively impact the progeny, altering different miRs and triggering different physiologicalalterations that could compromise offspring development. To investigate this, we exposed zebrafishmale siblings to a chronic stress protocol for 21 days. We performed RNA-sequencing (RNA-seq)analyses to identify differentially expressed small noncoding RNAs in 7-day postfertilization (dpf)larvae derived from paternally stressed males crossed with control females compared with the controlprogeny. We found a single miRNA differentially expressed—miR-29a—which was validated inlarva and was also tested in the sperm, testicles, and brain of the stressed progenitors. We observeda vertical transmission of chronic stress to the unexposed larvae, reporting novel consequences ofpaternally inherited chronic stress at a molecular level. The deregulation of mi-R29a in those larvaecould affect relevant biological processes affecting development, morphogenesis, or neurogenesis,among others. Additionally, these disruptions were associated with reduced rates of survival andhatching in the affected offspring.
PB MDPI
LK https://hdl.handle.net/10612/17465
UL https://hdl.handle.net/10612/17465
NO Riesco, M. F., Valcarce, D. G., Sellés-Egea, A., Esteve-Codina, A., Herráez, M. P., & Robles, V. (2023). miR-29a Is Downregulated in Progenies Derived from Chronically Stressed Males. International journal of molecular sciences, 24(18), 14107. https://doi.org/10.3390/ijms241814107
NO El artículo pertenece al special issue Ovary and Testis: Molecular Biological Insights
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 07-jun-2024