RT info:eu-repo/semantics/article
T1 Natural feed after weaning improves the reproductive status of "Solea senegalensis" breeders
A1 Martín, Ignacio
A1 Fernández Riesco, Marta
A1 Chaves Pozo, Elena
A1 Rodríguez, Cristina
A1 Martínez-Vázquez, Juan Manuel
A1 Robles Rodríguez, Vanesa
A1 Chereguini, María del Olvido
A1 Rasines, Inmaculada
A2 Bioquimica y Biologia Molecular
K1 Biología
K1 Senegalese sole
K1 Reproduction
K1 Sperm quality
K1 Production
K1 Feeding
K1 2415 Biología Molecular
K1 2401.19 Zoología Marina
AB [EN] The aim of this study was to evaluate the effect of long term natural feeding in the subsequent reproductivestatus of 4 years old cultured Solea senegalensis, and to determine if the potential changes were structural orfeeding dependent. To this aim, two different feeding regimes were used from one year after weaning and duringthe following 3 years; 1) a commercial dry food diet and 2) a natural feeding regime. After this period, theproportion of fluent males and the evolution of maturity stages of females over a breeding season were studied. Acomplete sperm quality analysis assessment was carried out, including individual volumes, motility, density andcurvilinear, rectilinear and mean velocities of spermatozoa. Moreover, viability and apoptosis indexes wereanalyzed as indicator of molecular sperm membrane integrity. Additionally, a morphological characterization ofthe testes during the spawning season was conducted. Finally, both groups were fed with the same commercialpellets during one year to evaluate the effect of the diet of previous years on sperm quality. The results of thisstudy showed how feeding can improve not only sperm quality and quantity, but also the proportion of fluentmales and females in advanced maturity stages. All the sperm quality parameters resulted significantly higher inthe group fed with a natural diet. Moreover, the number of apoptotic cells was significantly higher in the groupfed with a commercial diet. According to the morphological features of the testes, the animals fed with a naturaldiet presented more basal position, less protuberances and irregular edges when compared with the animals fedwith commercial diet. Interestingly, the progression of the spermatogenesis determined by the proportion ofgerm cells and the production of spermatozoa determined by the wider of the ducts system was also significantlylarger in the natural diet group.After the standardization of the diets, mean volume per male and production of total motile cells were significantlyhigher in the group that was previously fed a natural diet, confirming structural improvements.
PB Elsevier
SN 0044-8486
LK https://hdl.handle.net/10612/17467
UL https://hdl.handle.net/10612/17467
NO Martín, I., Fernández Riesco, M., Chaves Pozo, E., Rodríguez, C., Martínez Vázquez, J.M., Robles Rodríguez, V., Chereguini, M. del O., Rasines, I. (2021). Natural feed after weaning improves the reproductive status of "Solea senegalensis" breeders. Aquaculture. 530. https://doi.org/10.1016/j.aquaculture.2020.735740
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 12-jun-2024