RT info:eu-repo/semantics/article
T1 Real-time PCR detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum
A1 Martín, María Teresa
A1 Cobos Román, Rebeca
A1 Martín, Laura
A1 López Enríquez, Lorena
A2 Microbiologia
K1 Biología
K1 Biotecnología
K1 Grapevine trunk diseases
K1 Petri disease
K1 Phaeomoniella chlamydospora
K1 qPCR detection
K1 Phaeoacremonium minimum
K1 2414.06 Hongos
K1 2417.09 Fitopatología
AB [EN] Phaeomoniella chlamydospora and Phaeoacremonium aleophilum are the two main fungal causal agents of Petri disease and esca. Both diseases cause significant economic losses to viticulturalists. Since no curative control measures are known, proactive defensive measures must be taken. An important aspect of current research is the development of sensitive and time-saving protocols for the detection and identification of these pathogens. Real-time PCR based on the amplification of specific sequences is now being used for the identification and quantification of many infective agents. The present work reports real-time PCR protocols for identification of P. chlamydospora and P. aleophilum. Specificity was demonstrated against purified DNA from 60 P. chlamydospora isolates or 61 P. aleophilum isolates, and no amplification was obtained with 54 nontarget DNAs. The limits of detection (i.e., DNA detectable in 95% of reactions) were around 100 fg for P. chlamydospora and 50 fg for P. aleophilum. Detection was specific and sensitive for P. chlamydospora and P. aleophilum. Spores of P. chlamydospora and P. aleophilum were detected without the need for DNA purification. The established protocols detected these fungi in wood samples after DNA purification. P. chlamydospora was detectable without DNA purification and isolation in 67% of reactions. The detection of these pathogens in wood samples has great potential for use in pathogen-free certification schemes
PB American Society for Microbiology (ASM)
SN 0099-2240
LK https://hdl.handle.net/10612/17830
UL https://hdl.handle.net/10612/17830
NO Martín, M. T., Cobos, R., Martín, L., & López-Enríquez, L. (2012). Real-time PCR detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum. Applied and Environmental Microbiology, 78(11), 3985-3991. https://doi.org/10.1128/AEM.07360-11
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 01-jun-2024