RT info:eu-repo/semantics/article
T1 Genome-wide transcriptome response of Streptomyces tsukubaensis to N-acetylglucosamine: effect on tacrolimus biosynthesis
A1 Ordóñez Robles, María
A1 Rodríguez García, Antonio
A1 Martín Martín, Juan Francisco
A2 Microbiologia
K1 Biotecnología
K1 Streptomyces tsukubensis
K1 Tacrolimus
K1 FK506
K1 N-acetylglucosamine
K1 Transcriptomics
K1 Antibiotics biosynthesis regulation
K1 2415.01 Biología Molecular de Microorganismos
K1 2414.01 Antibióticos
AB [EN] Chitin is the second most abundant carbohydrate biopolymer present in soils and is utilized by antibiotic–producing Streptomyces species. Its monomer, N-acetylglucosamine (GlcNAc), regulates the developmental program of the model organism Streptomyces coelicolor. GlcNAc blocks differentiation when growing on rich medium whilst it promotes development on poor culture media. However, it is unclear if the same GlcNAc regulatory profile observed in S. coelicolor applies also to other industrially important Streptomyces species. We report here the negative effect of GlcNAc on differentiation and tacrolimus (FK506) production by Streptomyces tsukubaensis NRRL 18488. Using microarrays technology, we found that GlcNAc represses the transcription of fkbN, encoding the main transcriptional activator of the tacrolimus biosynthetic cluster, and of ppt1, encoding a phosphopantheteinyltransferase involved in tacrolimus biosynthesis. On the contrary, GlcNAc stimulated transcription of genes related to amino acid and nucleotide biosynthesis, DNA replication, RNA translation, glycolysis and pyruvate metabolism. The results obtained support those previously reported for S. coelicolor, but some important differences were observed; for example genes involved in GlcNAc transport and metabolism and genes encoding transcriptional regulators such as crr, ptsI, nagE1, nagE2, nagB, chiA, chiJ, ngcE, dasR or atrA are not significantly induced in S. tsukubaensis by GlcNAc addition. Differences in the GlcNAc transport systems, in the physiology of S. tsukubaensis and S. coelicolor and/or the different composition of the culture media used are likely to be responsible for the discrepancies observed between these specie
PB Elsevier
SN 0944-5013
LK https://hdl.handle.net/10612/17948
UL https://hdl.handle.net/10612/17948
NO Ordóñez-Robles, M., Rodríguez-García, A., & Martín, J. F. (2018). Genome-wide transcriptome response of Streptomyces tsukubaensis to N-acetylglucosamine: effect on tacrolimus biosynthesis. Microbiological Research, 217, 14-22. https://doi.org/10.1016/J.MICRES.2018.08.014
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 07-jun-2024