RT info:eu-repo/semantics/article
T1 Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender
A1 Gardela, Jaume
A1 Ruiz Conca, Mateo
A1 Palomares, Anna
A1 Olvera Maneu, Sergi
A1 García Calvo, Laura
A1 Lopez Bejar, Manel
A1 Martínez Pastor, Felipe
A1 Álvarez Rodríguez, Manuel
A2 Biologia Celular
K1 Biología
K1 Antioxidants
K1 Semen
K1 Sperm motility
K1 ProAKAP4
AB The effectiveness of rabbit-sperm cryopreservation is still below average compared to other domestic species. After the sperm cryopreservation process, post-thawing parameters like motility and membrane integrity are significantly compromised. The use of new extender constituents is an approach that can be used to improve the effectiveness of cryopreservation. Accordingly, we used honey (1.25, 2.5, 5, and 10%), coenzyme Q10 (100 and 200 μM), and β-carotene/α-tocopherol (500 μM/620 μM and 250 μM/310 μM) as candidate components for rabbit-sperm extenders during cryopreservation. Ejaculates from commercial adult rabbit bucks (n = 5) were cryopreserved using conventional freezing. Several post-thawing sperm parameters were assessed, including total motility, membrane integrity, viability, nuclear membrane integrity, acrosome reaction, and mitochondrial membrane potential and activation. Additionally, we performed hormonal analyses of the seminal plasma. Moreover, we analyzed the post-thawing levels of a molecular marker of sperm quality, proAKAP4, which was used in rabbits for the first time. Our findings showed that the 2.5% honey supplementation increased the post-thawing sperm motility (13.75 $\pm$ 3.75%) compared to the greater concentrations employed. However, the post-thawing motility was negatively affected by the coenzyme Q10 (0%, in both groups) but was not affected by the β-carotene/α-tocopherol supplementation (22 $\pm$ 18.15%, and 11.67 $\pm$ 10.17%). In conclusion, the cryopreservation protocols of this study did not help to maintain the sperm parameters after thawing. Further studies are required to identify novel protocols to mitigate the damage caused to rabbit sperm during cryopreservation.
PB MDPI
LK https://hdl.handle.net/10612/18048
UL https://hdl.handle.net/10612/18048
NO Gardela, J., Ruiz-Conca, M., Palomares, A., Olvera-Maneu, S., García-Calvo, L., López-Béjar, M., Martínez-Pastor, F., & Álvarez-Rodríguez, M. (2023). Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender. Animals: An Open Access Journal from MDPI, 13(14), 2392. https://doi.org/10.3390/ani13142392
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 18-jun-2024