RT info:eu-repo/semantics/article
T1 Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate
A1 Lombó Alonso, Marta
A1 González Rojo, Silvia
A1 Fernández Díez, Cristina
A1 Herráez Ortega, María Paz
A2 Biologia Celular
K1 Biología
K1 Bisphenol A
K1 Epigallocatechin Gallate
K1 Cardiotoxicity
K1 Histone acetylation
K1 Cardiogenesis
K1 2407 Biología Celular
K1 3214 Toxicología
K1 2401.07 Embriología Animal
K1 2409.99 Otros (Epigenética)
AB [EN] Exposure to the emerging contaminant bisphenol A (BPA) is ubiquitous and associated with cardiovascular disorders. BPA effect as endocrine disruptor is widely known but other mechanisms underlying heart disease, such as epigenetic modifications, remain still unclear. A compound of green tea, epigallocatechin gallate (EGCG), may act both as anti-estrogen and as inhibitor of some epigenetic enzymes. The aims of this study were to analyze the molecular processes related to BPA impairment of heart development and to prove the potential ability of EGCG to neutralize the toxic effects caused by BPA on cardiac health. Zebrafish embryos were exposed to 2000 and 4000 μg/L BPA and treated with 50 and 100 μM EGCG. Heart malformations were assessed at histological level and by confocal imaging. Expression of genes involved in cardiac development, estrogen receptors and epigenetic enzymes was analyzed by qPCR whereas epigenetic modifications were evaluated by whole mount immunostaining. BPA embryonic exposure led to changes in cardiac phenotype, induced an overexpression of hand2, a crucial factor for cardiomyocyte differentiation, increased the expression of estrogen receptor (esr2b), promoted an overexpression of a histone acetyltransferase (kat6a) and also caused an increase in histone acetylation, both mechanisms being able to act in sinergy. EGCG treatment neutralized all the molecular alterations caused by BPA, allowing the embryos to go on with a proper heart development. Both molecular mechanisms of BPA action (estrogenic and epigenetic) likely lying behind cardiogenesis impairment were successfully counteracted by EGCG treatment
PB Elsevier
SN 0269-7491
LK https://hdl.handle.net/10612/18117
UL https://hdl.handle.net/10612/18117
NO Lombó, M., González-Rojo, S., Fernández-Díez, C., & Herráez, M. P. (2019). Cardiogenesis impairment promoted by bisphenol A exposure is successfully counteracted by epigallocatechin gallate. Environmental Pollution, 246, 1008-1019. https://doi.org/10.1016/J.ENVPOL.2019.01.004
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 14-jun-2024