RT info:eu-repo/semantics/article
T1 Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
A1 Montes-Garrido, Rafael
A1 Fernández Riesco, Marta
A1 Anel López, Luis
A1 Neila Montero, Marta
A1 Palacín Martínez, Cristina
A1 Boixo, Juan Carlos
A1 Paz Cabello, Paulino de
A1 Ortega Ferrusola, Cristina
A1 Hassan, Mohamed A. A.
A1 Anel Rodríguez, Luis
A1 Álvarez García, Mercedes
A2 Medicina y Cirugia Animal
K1 Veterinaria
K1 Doppler
K1 Fertility
K1 Ovine
K1 Semen collection frequency
K1 Sperm quality
K1 Ultrasonography
K1 3109 Ciencias Veterinarias
K1 3104.11 Reproducción
AB [EN] The frequency of semen collection is a crucial factor to consider in the rams performance inside breeding centers workout. To evaluate this factor, ram Breeding Soundness Evaluation could include sperm quality evaluation and new predictive and non-invasive tools such as ultrasound technique. In this work, an advanced ultrasonography technology, analyzing the testicular volume, echotexture, and vascular function, was used in three different frequencies of semen collection (abstinence frequency, AF; standard frequency, SF; and intensive frequency, IF). Semen samples were cooled (15◦C, 6 h) and evaluated in terms of production, motility, viability, apoptosis, and content of reactive oxygen species. Correlation coe cients were calculated between ultrasonography measurements of echotexture and blood flow and sperm quality parameters. Our results showed an increase in the testicular echotexture when the frequency of semen collection was intensified. Doppler parameters (PSV, RI, PI, TABF) increased (P ≤ 0.05) when the frequency of semen collection was intensified. The sperm motility and functionality decreased in the samples of IF (P ≤ 0.05), evidencing the frequency of semen collection’s influence. Moreover, moderate positive correlations were established among echotexture and different Doppler parameters with motility parameters in SF. Furthermore, the influence of abstinence days on AI success was analyzed in a field assay. The highest fertility rates were obtained when males had two to five abstinence days. To conclude, frequency of semen collection could be influenced in terms of semen quantity and sperm quality, showing changes in parenchyma echotexture and testicular vascularization. The standard semen collection frequency was the most adequate option. In addition, ultrasonography may be a predictive tool for estimating variations in the sperm quality of donor rams subjected to different frequencies of semen collection in reproduction centers.
PB Frontiers
LK https://hdl.handle.net/10612/18207
UL https://hdl.handle.net/10612/18207
NO Montes-Garrido, R., Riesco, M. F., Anel-Lopez, L., Neila-Montero, M., Palacin-Martinez, C., Boixo, J. C., de Paz, P., Ortega-Ferrusola, C., Hassan, M. A. A., Anel, L., & Alvarez, M. (2022). Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams. Frontiers in Veterinary Science, 9. https://doi.org/10.3389/FVETS.2022.1035036
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 15-jun-2024