RT info:eu-repo/semantics/article
T1 Detection and genetic characterization of enteric viruses in diarrhoea outbreaks from swine farms in Spain
A1 Puente Fernández, Héctor
A1 Argüello Rodríguez, Héctor
A1 Cortey, Martí
A1 Gómez García, Manuel
A1 Mencía Ares, Óscar
A1 Pérez Pérez, Lucía
A1 Díaz, Iván
A1 Carvajal Urueña, Ana María
A2 Sanidad Animal
K1 Veterinaria
K1 Swine
K1 Multiplex RT-PCR
K1 Phylogenetic analysis
K1 Porcine astrovirus
K1 Porcine kobuvirus
K1 Porcine torovirus
K1 Mammalian orthoreovirus
K1 Porcine mastadenovirus
K1 3109 Ciencias Veterinarias
AB [EN] Background The aim of this work was to study the prevalence and distribution of Porcine astrovirus (PAstV), Porcinekobuvirus (PKoV), Porcine torovirus (PToV), Mammalian orthoreovirus (MRV) and Porcine mastadenovirus (PAdV) as wellas their association with widely recognized virus that cause diarrhoea in swine such as coronavirus (CoVs) and rotavirus(RVs) in diarrhoea outbreaks from Spanish swine farms. Furthermore, a selection of the viral strains was geneticallycharacterized.Results PAstV, PKoV, PToV, MRV and PAdV were frequently detected. Particularly, PAstV and PKoV were detected inalmost 50% and 30% of the investigated farms, respectively, with an age-dependent distribution; PAstV was mainlydetected in postweaning and fattening pigs, while PKoV was more frequent in sucking piglets. Viral co-infectionswere detected in almost half of the outbreaks, combining CoVs, RVs and the viruses studied, with a maximum of 5 differentviral species reported in three investigated farms. Using a next generation sequencing approach, we obtaineda total of 24 ARN viral genomes (> 90% genome sequence), characterizing for first time the full genome of circulatingstrains of PAstV2, PAstV4, PAstV5 and PToV on Spanish farms. Phylogenetic analyses showed that PAstV, PKoV and PToVfrom Spanish swine farms clustered together with isolates of the same viral species from neighboring pig producingcountries.Conclusions Although further studies to evaluate the role of these enteric viruses in diarrhoea outbreaks arerequired, their wide distribution and frequent association in co-infections cannot be disregard. Hence, their inclusioninto routine diagnostic panels for diarrhoea in swine should be considered.
PB BMC
LK https://hdl.handle.net/10612/18467
UL https://hdl.handle.net/10612/18467
NO Puente, H., Arguello, H., Cortey, M., Gómez-García, M., Mencía-Ares, O., Pérez-Perez, L., Díaz, I., & Carvajal, A. (2023). Detection and genetic characterization of enteric viruses in diarrhoea outbreaks from swine farms in Spain. Porcine Health Management, 9(1). https://doi.org/10.1186/S40813-023-00326-W
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 07-jun-2024