RT info:eu-repo/semantics/article T1 Occurrence of Leishmaniasis in Iberian Wolves in Northwestern Spain A1 Merino Goyenechea, Javier A1 Castilla Gómez de Agüero, Verónica A1 Palacios Alberti, Jesús A1 Balaña Fouce, Rafael A1 Martínez Valladares, María A1 A2 Sanidad Animal K1 Sanidad animal K1 Wild fauna K1 Iberian wolf K1 Canine leishmaniasis K1 Diagnosis K1 PCR K1 Buccal samples K1 Hair K1 Northwestern Spain K1 3109 Ciencias Veterinarias AB [EN] Canine leishmaniasis is an important vector-borne protozoan disease in dogs that is responsible for serious deterioration in their health. In the Iberian Peninsula, as in most countries surrounding the Mediterranean Sea, canine leishmaniasis is caused by Leishmania infantum (zymodeme MON-1), a digenetic trypanosomatid that harbors in the parasitophorous vacuoles of host macrophages, causing severe lesions that can lead to death if the animals do not receive adequate treatment. Canine leishmaniasis is highly prevalent in Spain, especially in the Mediterranean coastal regions (Levante, Andalusia and the Balearic Islands), where the population of domestic dogs is very high. However, the presence of this disease has been spreading to other rural and sparsely populated latitudes, and cases of leishmaniasis have been reported for years in wildlife in northwestern Spain. This work describes for the first time the presence of wolves that tested positive for leishmaniasis in the vicinity of the Sierra de la Culebra (Zamora province, northwestern Spain), a protected sanctuary of this canid species, using PCR amplification of L. infantum DNA from different non-invasive samples such as buccal mucosa and those from both ears and hair. In addition to live animals (21), samples from carcasses of mainly roadkill animals (18) were also included and analyzed using the same technique, obtaining a positivity rate of 18 of the 39 wolves sampled (46.1%) regardless of their origin PB MDPI LK https://hdl.handle.net/10612/18618 UL https://hdl.handle.net/10612/18618 NO Merino Goyenechea, J., Castilla Gómez de Agüero, V., Palacios Alberti, J., Balaña Fouce, R., & Martínez Valladares, M. (2023). Occurrence of Leishmaniasis in Iberian Wolves in Northwestern Spain. Microorganisms, 11(5). https://doi.org/10.3390/MICROORGANISMS11051179 DS BULERIA. Repositorio Institucional de la Universidad de León RD 06-jun-2024