RT info:eu-repo/semantics/article
T1 Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping
A1 Ramírez, Hugo
A1 Echeverría, Irache
A1 Benito, Alfredo A.
A1 Glaria, Idoia
A1 Benavides, Julio
A1 Pérez Pérez, Valentín
A1 de Andrés, Damián
A1 Reina, Ramsés
A2 Sanidad Animal
K1 Sanidad animal
K1 Small ruminant lentiviruses
K1 TMEM154
K1 ELISA
K1 PCR
K1 3109 Ciencias Veterinarias
AB [EN] Small ruminant lentiviruses (SRLV) cause an incurable multiorganic disease widely spread in sheep and goats that disturbs animal welfare and production. In the absence of a vaccine, control measures have been traditionally based on early diagnosis and breeding with virus-inactivated colostrum with segregation of seropositive animals. However, antigenic heterogeneity, poor antibody production due to low viral load, and single strain design of most available ELISA, pose a threat to SRLV diagnosis. Genome-wide association studies have described TMEM154 E35K polymorphism as a good genetic marker for selection of resistant animals in some American and European breeds. In this study, a multitargeted serological and virological screening of more than 500 animals from four different breeds (latxa, raza Navarra, assaf, and churra) attending to SRLV infection status was performed. Then, animals were genotyped to characterize TMEM154 E35K polymorphism. ELISA procedures, individually considered, only identified a proportion of the seropositive animals, and PCR detected a fraction of seronegative animals, globally offering different animal classifications according to SRLV infection status. TMEM154 allele frequency differed substantially among breeds and a positive association between seroprevalence and TMEM154 genotype was found only in one breed. Selection based on TMEM154 may be suitable for specific ovine breeds or SRLV strains, however generalization to the whole SRLV genetic spectrum, ovine breeds, or epidemiological situation may need further validation
PB MDPI
LK https://hdl.handle.net/10612/18836
UL https://hdl.handle.net/10612/18836
NO Ramírez, H., Echeverría, I., Benito, A. A., Glaria, I., Benavides, J., Pérez, V., de Andrés, D., & Reina, R. (2021). Accurate diagnosis of small ruminant lentivirus infection is needed for selection of resistant sheep through tmem154 e35k genotyping. Pathogens, 10(1), 1-16. https://doi.org/10.3390/PATHOGENS10010083
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 05-jun-2024