RT info:eu-repo/semantics/article
T1 Early response of monocyte-derived macrophages from vaccinated and non-vaccinated goats against in vitro infection with Mycobacterium avium subsp. paratuberculosis
A1 Arteche Villasol, Noive
A1 Gutiérrez Expósito, Daniel
A1 Vallejo, Raquel
A1 Espinosa Cerrato, José
A1 Elguezabal, Natalia
A1 Ladero Auñón, Iraia
A1 Royo, Marcos
A1 Ferreras Estrada, María Del Carmen
A1 Benavides, Julio
A1 Pérez Pérez, Valentín
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Map
K1 Vaccination
K1 Caprine monocyte-derived macrophages
K1 Viability
K1 Phagocytosis
K1 3109 Ciencias Veterinarias
K1 3109.05 Microbiología
K1 3109.05 Microbiología
K1 2412.10 Vacunas
AB [EN]Paratuberculosis is a disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). Vaccination is the most cost-effective control method. However, despite the fact that macrophages are the main target cells for this pathogen, the precise mechanisms behind the response of the macrophage to Map infection and how it is modified by vaccination are yet poorly understood. The aim of this study was to investigate the effect of Silirum® vaccination in the early immune response of caprine monocyte-derived macrophages (CaMØs). Peripheral blood mononuclear cells (PBMCs) were obtained from vaccinated and non-vaccinated goats, cultured in vitro until differentiation to macrophages and infected with Map. After a 24 h incubation, Map viability and DNA were assessed in culture by viable colony count and real time quantitative polymerase chain reaction (qPCR). In addition, Map phagocytosis and expression of IL-10, IL-12, IFN-γ, TNF-α, IL-17A, IL-1β, iNOS, IL-6 and MIP-1β were also evaluated through immunofluorescence labelling and reverse transcriptase qPCR (RT-qPCR), respectively. A significant reduction of Map viability was observed in both supernatants (P < 0.05) and CaMØs (P < 0.001) from the vaccinated group. Similarly, the percentage of infected CaMØs and the number of internalized Map by CaMØs (P < 0.0001) was higher in the vaccinated group. Finally, iNOS (P < 0.01) and IL-10 were significantly up-regulated in CaMØs from vaccinated goats, whereas only MIP-1β was up-regulated in non-vaccinated animals (P < 0.05). These results show that vaccination modifies the immune response of CaMØs, suggesting that the phagocytosis and microbiocidal activity of macrophages against Map is enhanced after vaccination.
PB BMC
LK https://hdl.handle.net/10612/18880
UL https://hdl.handle.net/10612/18880
NO Arteche-Villasol, N., Gutiérrez-Expósito, D., Vallejo, R., Espinosa, J., Elguezabal, N., Ladero-Auñon, I., Royo, M., Ferreras Estrada, M. d. C., Benavides, J., & Pérez, V. (2021). Early response of monocyte-derived macrophages from vaccinated and non-vaccinated goats against in vitro infection with Mycobacterium avium subsp. paratuberculosis. Veterinary Research, 52(1). https://doi.org/10.1186/S13567-021-00940-Y
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 01-jun-2024