RT info:eu-repo/semantics/article
T1 Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
A1 Valente, Angela H.
A1 de Roode, Matthew
A1 Ernst, Madeleine
A1 Peña Espinoza, Miguel
A1 Bornancin, Louis
A1 Bonde, Charlotte S.
A1 Martínez Valladares, María
A1 Ramünke, Sabrina
A1 Krücken, Jürgen
A1 Simonsen, Henrik T.
A1 Thamsborg, Stig M.
A1 Williams, Andrew R.
A2 Sanidad Animal
K1 Sanidad animal
K1 Chicory
K1 Anthelmintic
K1 Parasite
K1 Sesquiterpene lactones
K1 3109 Ciencias Veterinarias
AB [EN] Increasing resistance towards anthelmintic drugs has necessitated the search for alternative treatments for the control of gastrointestinal nematode parasites. Animals fed on chicory (Cichorium intybus L.), a temperate (pasture) crop, have reduced parasite burdens, hence making C. intybus a potentially useful source for novel anthelmintic compounds or a diet-based preventive/therapeutic option. Here, we utilized in vitro bioassays with the parasitic nematode Ascaris suum and molecular networking techniques with five chicory cultivars to identify putative active compounds. Network analysis predicted sesquiterpene lactones (SL) as the most likely group of anthelmintic compounds. Further bioassay-guided fractionation supported these predictions, and isolation of pure compounds demonstrated that the SL 8-deoxylactucin (8-DOL) is the compound most strongly associated with anti-parasitic activity. Furthermore, we showed that 8-DOL acts in a synergistic combination with other SL to exert the anti-parasitic effects. Finally, we established that chicory-derived extracts also showed activity against two ruminant nematodes (Teladorsagia circumcincta and Cooperia oncophora) in in vitro assays. Collectively, our results confirm the anti-parasitic activity of chicory against a range of nematodes, and pave the way for targeted extraction of active compounds or selective breeding of specific cultivars to optimize its future use in human and veterinary medicine
PB Elsevier
SN 2211-3207
LK https://hdl.handle.net/10612/18882
UL https://hdl.handle.net/10612/18882
NO Valente, A. H., de Roode, M., Ernst, M., Peña-Espinoza, M., Bornancin, L., Bonde, C. S., Martínez-Valladares, M., Ramünke, S., Krücken, J., Simonsen, H. T., Thamsborg, S. M., & Williams, A. R. (2021). Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation. International Journal for Parasitology: Drugs and Drug Resistance, 15, 105-114. https://doi.org/10.1016/J.IJPDDR.2021.02.002
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 02-jun-2024