RT info:eu-repo/semantics/article
T1 Bioaugmentation Treatment of a PAH-Polluted Soil in a Slurry Bioreactor
A1 Forján, Rubén
A1 Lores, Iván
A1 Baragaño, Diego
A1 Gallego, José Luis R.
A1 Peláez, Ana Isabel
A1 Sierra Fernández, Carlos
A2 Ingeniería CartograficaGeodesica y Fotogrametria
K1 Ingenierías
K1 Ingeniería de minas
K1 Pah
K1 Bioslurry
K1 Soil Pollution
K1 Bioaugmentation
K1 Pseudomonas
K1 Rhodococcus
AB [EN] A bioslurry reactor was designed and used to treat loamy clay soil polluted with polycyclicaromatic hydrocarbons (PAHs). To this end, biostimulation alone, or combined with bioaugmentationwith two bacterial strains (Rhodocccus erythropolis and Pseudomonas stuzeri) previously isolated fromthe polluted site, was applied. The PAH concentrations decreased notably after 15 days in allof the treatments. The concentrations of the two- and three-ring compounds fell by >80%, and,remarkably, the four- to six-ring PAHs also showed a marked decrease (>70%). These results thusindicate the capacity of bioslurry treatments to improve, notably, the degradation yields obtained in aprevious real-scale remediation carried out using biopiles. In this sense, the remarkable results forrecalcitrant PAHs can be attributed to the increase pollutants’ bioavailability achieves in the slurrybioreactors. Regarding bioaugmentation, although treatment with R. erythropolis led to a somewhatgreater reduction of lighter PAHs at 15 days, the most time-effective treatment was achieved usingP. stutzeri, which led to an 84% depletion of total PAHs in only three days. The effects of microbialdegradation of other organic compounds were also monitored by means of combined qualitative andquantitative gas chromatography mass spectrometry (GC–MS) tools, as was the evolution of microbialpopulations, which was analyzed by culture and molecular fingerprinting experiments. On the basisof our findings, bioslurry technology emerges as a rapid and operative option for the remediation ofpolluted sites, especially for fine soil fractions with a high load of recalcitrant pollutants.
PB MDPI
LK https://hdl.handle.net/10612/19249
UL https://hdl.handle.net/10612/19249
NO Forján, R.; Lores, I.; Baragaño, D.; Gallego, J. L. R.; Peláez, A. I.; Sierra Fernández, C. (2020). Bioaugmentation Treatment of a PAH-Polluted Soil in a Slurry Bioreactor. Applied Sciences, 10(8), https://doi.org/10.3390/APP10082837
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 03-jun-2024