RT info:eu-repo/semantics/article
T1 Characterization of Antimicrobial Resistance Determinants and Class 1 and Class 2 Integrons in Salmonella enterica spp., Multidrug-Resistant Isolates from Pigs
A1 Argüello Rodríguez, Héctor
A1 Guerra, Beatriz
A1 Rodríguez, Irene
A1 Rubio, Pedro
A1 Carvajal Urueña, Ana María
A2 Sanidad Animal
K1 Sanidad animal
K1 Resistance gene
K1 Swine
K1 Food-borne pathogen
K1 Public health
K1 Antibiotic
K1 3109 Ciencias Veterinarias
AB [EN] Antimicrobial resistance (AMR) and Salmonella spp., are primary concerns in public health. The present study characterizes the AMR determinants of 62 multi-drug resistant (MDR) Salmonella enterica spp., isolates from swine, which were obtained between 2004-2006, a major source of human salmonellosis. The AMR determinants were investigated by PCR, checking the presence of class 1 and class 2 integrons and 29 resistance genes. Genes sul1, blaTEM1-like, aadA2, tet(A), and dfrA12 were more prevalent (p < 0.05) within the determinants that were checked for each of these antimicrobials. Co-existence of different genes conferring resistance to the same antimicrobial was common. No differences in AMR determinants prevalence were observed between Salmonella Typhimurium and other serovars from the study. Class 1 integrons were detected in 48 of 62 isolates, again with no differences being linked to any serovar. Nine different variable regions were observed, 1000 bp/aadA2-1200 bp/blaPSE-1 (13 isolates) and blaOXA-like/aadA1 (eight isolates) were the most common. Four isolates, including S. Typhimurium (2), Salmonella Bredeney (1), and Salmonella Kapemba (1) harboured a class 2 integron 2300 bp estX-sat2-aadA1. Results from the study highlight the importance of class 1 integrons and certain genes in MDR swine Salmonella isolates. The information is of relevance for monitoring in the forthcoming scope of reduction of antibiotic usage in swine production
PB MDPI
LK https://hdl.handle.net/10612/19346
UL https://hdl.handle.net/10612/19346
NO Argüello, H., Guerra, B., Rodríguez, I., Rubio, P., & Carvajal, A. (2018). Characterization of antimicrobial resistance determinants and class 1 and class 2 integrons in salmonella enterica spp., multidrug-resistant isolates from pigs. Genes, 9(5). https://doi.org/10.3390/GENES9050256
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 07-jun-2024