RT info:eu-repo/semantics/article
T1 A serosurvey of selected cystogenic coccidia in Spanish equids: first detection of anti-Besnoitia spp. specific antibodies in Europe
A1 Gutiérrez Expósito, Daniel
A1 García Bocanegra, Ignacio
A1 Howe, Daniel K.
A1 Arenas Montes, Antonio
A1 Yeargan, Michelle R.
A1 Ness, SallyAnne L.
A1 Ortega Mora, Luis Miguel
A1 Álvarez García, G.
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Besnoitia spp.
K1 Sarcocystis spp.
K1 Neospora spp.
K1 Spain
K1 Serosurvey
K1 Horse
K1 Donkey
K1 Mule
K1 Risk factors
K1 3109 Ciencias Veterinarias
K1 2401.12 Parasitología Animal
K1 3104.05 Équidos
AB [EN]Background: Equine besnoitiosis, caused by Besnoitia bennetti, and equine protozoal myeloencephalitis (EPM), caused by Sarcocystis neurona and Neospora hughesi are relevant equine diseases in the Americas that have been scarcely studied in Europe. Thus, a serosurvey of these cystogenic coccidia was carried out in Southern Spain. A cross-sectional study was performed and serum samples from horses (n=553), donkeys (n=85) and mules (n=83) were included. An in-house enzyme-linked immunosorbent assay (ELISA) was employed to identify a Besnoitia spp. infection and positive results were confirmed by an a posteriori western blot. For Neospora spp. and Sarcocystis spp., infections were detected using in-house ELISAs based on the parasite surface antigens N. hughesi rNhSAG1 and S. neurona rSnSAG2/3/4. Risk factors associated with these protozoan infections were also investigated. Results: Antibodies against Besnoitia spp., Neospora spp. and Sarcocystis spp. infections were detected in 51 (7.1%), 46 (6.4%) and 20 (2.8%) of 721 equids, respectively. The principal risk factors associated with a higher seroprevalence of Besnoitia spp. were the host species (mule or donkey), the absence of shelter and the absence of a rodent control programme. The presence of rodents was the only risk factor for Neospora spp. infection. Conclusions: This study was the first extensive serosurvey of Besnoitia spp. infection in European equids accomplished by two complementary tests and gives evidence of the presence of specific antibodies in these populations. However, the origin of the infection is still unclear. Further parasite detection and molecular genotyping are needed to identify the causative Besnoitia and Neospora species. Finally, cross-reactions with antibodies directed against other species of Sarcocystis might explain the positive reactions against the S. neurona antigens.
PB BMC
LK https://hdl.handle.net/10612/19719
UL https://hdl.handle.net/10612/19719
NO Gutiérrez-Expósito, D., García-Bocanegra, I., Howe, D. K., Arenas-Montes, A., Yeargan, M. R., Ness, S. L., Ortega-Mora, L. M., & Álvarez-García. (2017). A serosurvey of selected cystogenic coccidia in Spanish equids: First detection of anti-Besnoitia spp. specific antibodies in Europe. BMC Veterinary Research, 13, Article e128. https://doi.org/10.1186/S12917-017-1046-Z
NO © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 07-jun-2024