RT info:eu-repo/semantics/article
T1 Vaccination sequence effects on immunological response and tissue bacterial burden in paratuberculosis infection in a rabbit model
A1 Arrazuria, Rakel
A1 Molina, Elena
A1 Garrido, Joseba M.
A1 Pérez Pérez, Valentín
A1 Juste, Ramon A.
A1 Elguezabal, Natalia
A2 Sanidad Animal
K1 Sanidad animal
K1 Immunology
K1 Paratuberculosis
K1 Rabbits
K1 Vaccination
K1 3109 Ciencias Veterinarias
AB [EN] Paratuberculosis (PTB), a chronic granulomatous enteritis produced by Mycobacterium avium subspecies paratuberculosis (MAP), is considered as one of the diseases with the highest economic impact in the ruminant industry. Vaccination against MAP is recommended during the first months after birth on the basis that protection would be conferred before the first contact with mycobacteria. However, little is known about the therapeutic effect of MAP vaccination in controlled experimental conditions. The current study was designed to evaluate the efficacy of vaccination before and after challenge with MAP in a rabbit infection model. The rabbits were divided into four groups: non-infected control (NIC, n = 4), infected control challenged with MAP (IC, n = 5), vaccinated and challenged 1 month after with MAP (VSI, n = 5) and challenged with MAP and vaccinated 2 months later (IVS, n = 5). The results from this study show a quick increase in IFN-γ release upon stimulation with bovine, avian and johnin PPD in animals vaccinated before MAP challenge. All vaccinated animals show an increased humoral response as seen by western blot and ELISA. The final bacteriology index (considering tissue culture and qPCR) shows that the IC group was the most affected. Vaccination after infection (IVS) produced the lowest bacteriology index showing significant differences with the IC group (p = 0.034). In conclusion, vaccination against MAP shows positive effects in a rabbit model. However, vaccination after infection shows a slightly stronger protective effect compared to vaccination before infection, suggesting a therapeutic effect. This feature could be applied to previously infected adult animals under field conditions
PB BMC
LK https://hdl.handle.net/10612/19907
UL https://hdl.handle.net/10612/19907
NO Arrazuria, R., Molina, E., Garrido, J. M., Pérez, V., Juste, R. A., & Elguezabal, N. (2016). Vaccination sequence effects on immunological response and tissue bacterial burden in paratuberculosis infection in a rabbit model. Veterinary Research, 47(1). https://doi.org/10.1186/S13567-016-0360-Y
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 18-jun-2024