RT info:eu-repo/semantics/article
T1 Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
A1 Silva, Vanessa
A1 Correira, Elisete
A1 Pereira, José Eduardo
A1 González Machado, Camino
A1 Capita González, Rosa María
A1 Alonso Calleja, Carlos
A1 Igrejas, Gilberto
A1 Poeta, Patrícia
A2 Nutricion y Bromatologia
K1 Tecnología de los alimentos
K1 Biofilm
K1 Staphylococci
K1 Coagulase-negative staphylococci (CoNS)
K1 2414.04 Bacteriología
K1 3309 Tecnología de Los Alimentos
K1 3109.05 Microbiología
AB [EN] The ability of biofilm formation seems to play an important role in the virulence of staphylococci. However, studies reporting biofilm formation of coagulase-negative staphylococci isolated from animals are still very scarce. Thus, we aimed to evaluate the biofilm-forming capacity of CoNS and S. pseudintermedius isolated from several animal species and to investigate the effect of conventional antimicrobials on biofilm reduction. A total of 35 S. pseudintermedius and 192 CoNS were included. Biofilm formation was accessed by the microtiter plate assay and the biofilms were stained by crystal violet. Association between biofilm formation and staphylococci species and antimicrobial resistance was also performed. Biofilm susceptibility testing was performed with tetracycline and amikacin at the minimum inhibitory concentration (MIC) and 10 × MIC. The metabolic activity of the biofilm cells after antimicrobial treatment was accessed by the XTT assay. All isolates formed biofilm, with S. urealyticus producing the most biofilm biomass and S. pseudintermedius producing the least biomass. There was a positive association between biofilm formation and multidrug resistance as well as resistance to individual antimicrobials. Neither tetracycline nor amikacin were able to eradicate the biofilm, not even at the highest concentration used. This study provides new insights into biofilm formation and the effects of antimicrobials on CoNS species.
PB MDPI
LK https://hdl.handle.net/10612/20244
UL https://hdl.handle.net/10612/20244
NO Silva, V., Correia, E., Pereira, J. E., González Machado, C., Capita, R., Alonso Calleja, C., Igrejas, G., & Poeta, P. (2022). Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species. Pathogens, 11(6), Article e689. https://doi.org/10.3390/PATHOGENS11060689
NO Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 15-jun-2024