RT info:eu-repo/semantics/article
T1 Integrated analyses of the methylome and transcriptome to unravel sex differences in the perirenal fat from suckling lambs
A1 De Souza Fonseca, Pablo Augusto
A1 Alonso García, María
A1 Pelayo, Rocío
A1 Marina García, Héctor
A1 Esteban Blanco, Cristina
A1 Mateo Oyagüe, Javier
A1 Gutiérrez Gil, Beatriz
A1 Arranz Santos, Juan José
A1 Suárez Vega, Aroa
A2 Producción Animal
K1 Producción animal
K1 RNA sequencing
K1 Whole-genome bisulfite sequencing
K1 Suckling lambs
K1 Omics integration
K1 Fat deposits
K1 Perirenal fat
K1 3309 Tecnología de Los Alimentos
AB [EN] In sheep, differences were observed regarding fat accumulation and fatty acid (FA) composition between males and females, which may impact the quality and organoleptic characteristics of the meat. The integration of different omics technologies is a relevant approach for investigating biological and genetic mechanisms associated with complex traits. Here, the perirenal tissue of six male and six female Assaf suckling lambs was evaluated using RNA sequencing and whole-genome bisulfite sequencing (WGBS). A multiomic discriminant analysis using multiblock (s)PLS-DA allowed the identification of 314 genes and 627 differentially methylated regions (within these genes), which perfectly discriminate between males and females. These candidate genes overlapped with previously reported QTLs for carcass fat volume and percentage of different FAs in milk and meat from sheep. Additionally, differentially coexpressed (DcoExp) modules of genes between males (nine) and females (three) were identified that harbour 22 of these selected genes. Interestingly, these DcoExp were significantly correlated with fat percentage in different deposits (renal, pelvic, subcutaneous and intramuscular) and were associated with relevant biological processes for adipogenesis, adipocyte differentiation, fat volume and FA composition. Consequently, these genes may potentially impact adiposity and meat quality traits in a sex-specific manner, such as juiciness, tenderness and flavour
PB Frontiers Media
LK https://hdl.handle.net/10612/20399
UL https://hdl.handle.net/10612/20399
NO Fonseca, P. A. S., Alonso García, M., Pelayo, R., Marina, H., Esteban Blanco, C., Mateo, J., Gutiérrez Gil, B., Arranz, J.-J., & Suárez-Vega, A. (2022). Integrated analyses of the methylome and transcriptome to unravel sex differences in the perirenal fat from suckling lambs. Frontiers in Genetics, 13. https://doi.org/10.3389/FGENE.2022.1035063
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 10-jun-2024