RT info:eu-repo/semantics/article
T1 Evaluation of two antimicrobial packaging films against Escherichia coli O157:H7 strains in vitro and during storage of a spanish ripened sheep cheese (zamorano)
A1 Otero, Verónica
A1 Becerril González, Raquel
A1 Santos Buelga, Jesús Ángel
A1 Rodríguez Calleja, José María
A1 Nerín, Cristina
A1 García López, María Luisa
A2 Nutricion y Bromatologia
K1 Tecnología de los alimentos
K1 Veterinaria
K1 E. coli O157:H7
K1 Ethyl Lauroyl Arginate
K1 LAE
K1 Antimicrobial
K1 Queso de oveja
K1 Essential oils
K1 Zamora
K1 Active packaging
AB Entidades financiadoras: Junta de Castilla y León (Ayuda Grupo de Excelencia GR155 y Proyecto de Investigación LE331A12-2). Proyecto de Investigación AGL2011-26118/ALI. The antimicrobial activity of two packaging films (polypropylene –PP- and polyethylene terephthalate –PET-) coated with different concentrations (0, 4, 6 and 8%) of essential oil from Origanum vulgare (OR) and Ethyl Lauroyl Arginate HCl (LAE) was tested against two E. coli O157:H7 strains using in vitro systems and a raw milk sheep cheese model (Zamorano). The influence of the antimicrobials on the sensory attributes of cheese was also evaluated. For both strains, the MBC (minimum bactericidal concentration) values were identical to their respective MIC (minimum inhibitory concentration) values and lower for LAE (25 mg/l) than for OR (200-400 mg/l). PP and PET films coated with OR were tested by a vapour phase assay and the Japanish Standard method (JIS Z 2801:2000). Both films coated with LAE were tested by an agar diffusion method. Overall, in vitro tests were effective against both strains. The inhibitory activity depended on the active compound concentration, the target strain and the packaging material, PET being more effective than PP. For inoculated cheese slices, OR and LAE PP films did not effectively decrease E. coli O157:H7 counts after 7-days cold storage. PET films incorporating 6 and 8 % of OR and LAE significantly (p< 0.05) decreased the numbers of both strains and also did 4% for the reference and wild strain depending on the antimicrobial. LAE PP, OR PET and LAE PET did not significantly (p> 0.05) affect sensorial characteristics of Zamorano cheese. Packaging with PET films coated with  6% LAE concentrations might be useful in reducing E. coli O157:H7 numbers in sheep cheese
PB Elsevier
YR 2015
FD 2015-02-25
LK http://hdl.handle.net/10612/4166
UL http://hdl.handle.net/10612/4166
NO Food Control, 42
NO P. 296-302
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-may-2024