RT info:eu-repo/semantics/article
T1 Differences in Haemophilus parasuis adherence to and invasion of AOC-45 porcine aorta endothelial cells
A1 Frandoloso, Rafael
A1 Pivato, Mateus
A1 Martínez Martínez, Sonia
A1 Rodríguez Ferri, Elías Fernando
A1 Kreutz, Luiz Carlos
A1 Gutiérrez Martín, César Bernardo
A2 Sanidad Animal
K1 Sanidad animal
K1 Haemophilus parasuis
K1 Glässer’s disease
K1 AOC-45 cells
K1 Invasión
AB Background: The pathogenesis of Haemophilus parasuis depends on the bacterium’s ability to interact withendothelial cells and invade adjacent tissues. In this study, we investigated the abilities of eight H. parasuisreference strains belonging to serovars 1, 2, 4, 5, 7, 9, 10 and 13 to adhere to and invade porcine aortic endothelialcells (AOC-45 cell line).Results: The strains belonging to serovars 1, 2 and 5 were able to attach at high rates between 60 and 240 min ofincubation, and serovars 4, 7 and 13 had moderate attachment rates; however, the strains belonging to serovars 9and 10 had low adherence at all time points. Strong adherence was observed by scanning electron microscopy forthe strains of serovars 5 and 4, which had high and moderate numbers, respectively, of H. parasuis cells attached toAOC-45 cells after 240 min of incubation. The highest invasiveness was reached at 180 min by the serovar 4 strain,followed by the serovar 5 strain at 240 min. The invasion results differed substantially depending on the strain.Conclusion: The reference strains of H. parasuis serovars 1, 2, 4 and 5 exhibited high adhesion and invasion levelsto AOC-45 porcine aorta endothelial cells, and these findings could aid to better explain the pathogenesis of thedisease caused by these serovars.
PB BioMed Central
YR 2017
FD 2017-07-26
LK http://hdl.handle.net/10612/6456
UL http://hdl.handle.net/10612/6456
NO BMC Veterinary Research, 2013, vol. 9, n. 207
NO 8 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024