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Título
Streptomyces tsukubaensis as a new model for carbon repression: transcriptomic response to tacrolimus repressing carbon sources
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Applied Microbiology and Biotechnology
Número de la revista
22
Cita Bibliográfica
Ordóñez-Robles, M., Santos-Beneit, F., Albillos, S.M., Liras, P., Martín, J. F., & Rodríguez-García, A. (2017) Streptomyces tsukubaensis as a new model for carbon repression: transcriptomic response to tacrolimus repressing carbon sources. Appl Microbiol Biotechnol 101(22), 8181–8195. https://doi.org/10.1007/s00253-017-8545-5
Editorial
Springer
Fecha
2017-10-05
ISSN
0175-7598
Resumen
[EN] In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production
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- info:eu-repo/grantAgreement/MICINN/Programa Nacional de Internacionalización de la I+D/PIM2010EEI-00677/DESARROLLO DE UN PROCESO DE FERMENTACION ROBUSTO PARA LA PRODUCCION DE TACROLIMUS E INMUNOSUPRESORES
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