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Título
Characterization and cross‐protection of experimental infections with SeCoV and two PEDV variants
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Transboundary and Emerging Diseases
Número de la revista
6
Cita Bibliográfica
Puente, H., Díaz, I., Arguello, H., Mencía-Ares, Ó., Gómez-García, M., Pérez-Pérez, L., Vega, C., Cortey, M., Martín, M., Rubio, P., & Carvajal, A. (2022). Characterization and cross-protection of experimental infections with SeCoV and two PEDV variants. Transboundary and Emerging Diseases, 69(6), 3225-3237. https://doi.org/10.1111/TBED.14674
Editorial
Wiley
Fecha
2022
ISSN
1865-1674
Resumen
[EN] The aim of this study was to characterize the infection of weaned pigs with swine enteric coronavirus (SeCoV) – a chimeric virus most likely originated from a recombination event between porcine epidemic diarrhoea virus (PEDV) and transmissible gastroenteritis virus, or its mutant porcine respiratory coronavirus – and two PEDV G1b variants, including a recently described recombinant PEDV-SeCoV (rPEDV-SeCoV), as well as to determine the degree of cross-protection achieved against the rPEDV-SeCoV. For this purpose, forty-eight 4-week-old weaned pigs were randomly allocated into four groups of 12 animals. Piglets within each group were primary inoculated with one of the investigated viral strains (B: PEDV; C: SeCoV and D: rPEDV-SeCoV) or mock-inoculated (A), and exposed to rPEDV-SeCOV at day 20 post-infection; thus, group A was primary challenged (-/rPEDV-SeCoV), groups B and C were subjected to a heterologous re-challenge (PEDV/rPEDV-SeCoV and SeCoV/rPEDV-SeCoV, respectively), and group D to a homologous re-challenge (rPEDV-SeCoV/rPEDV-SeCoV), Clinical signs, viral shedding, microscopic lesions and specific humoral and cellular immune responses (IgG, IgA, neutralizing antibodies and IgA and IFN-γ-secreting cells) were monitored. After primo-infection, all three viral strains induced an undistinguishable mild-to-moderate clinical disease with diarrhoea as the main sign and villus shortening lesions in the small intestine. In homologous re-challenged pigs, no clinical signs or lesions were observed, and viral shedding was only detected in a single animal. This fact may be explained by the significant high level of rPEDV-SeCoV-specific neutralizing antibodies found in these pigs before the challenge. In contrast, prior exposure to a different PEDV G1b variant or SeCoV only provided partial cross-protection, allowing rPEDV-SeCoV replication and shedding in faeces
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Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MECD/Programa Estatal de Promoción del Talento y su Empleabilidad/ FPU16/03485/ES/ / /
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