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Título
Protocol for bacterial typing using Fourier transform infrared spectroscopy
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
STAR Protocols
Número de la revista
2
Cita Bibliográfica
Yang, H., Shi, H., Feng, B., Wang, L., Chen, L., Alvarez-Ordóñez, A., Zhang, L., Shen, H., Zhu, J., Yang, S., Ding, C., Prietod, M., Yang, F., & Yu, S. (2023). Protocol for bacterial typing using Fourier transform infrared spectroscopy. STAR Protocols, 4(2), Article e102223. https://doi.org/10.1016/J.XPRO.2023.102223
Editorial
Cell Press
Fecha
2023
ISSN
2666-1667
Resumen
[EN]The Fourier transform infrared (FT-IR) signals obtained from bacterial samples are specific and reproducible, making FT-IR an efficient tool for bacterial typing at a subspecies level. However, the typing accuracy could be affected by many factors, including sample preparation and spectral acquisition. Here, we present a unified protocol for bacterial typing based on FT-IR spectroscopy. We describe sample preparation from bacterial culture and FT-IR spectrum collection. We then detail FT-IR spectrum preprocessing and multivariate analysis of spectral data for bacterial typing.
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