Study on Viability and Chondrogenic Differentiation of Cryopreserved Adipose Tissue-Derived Mesenchymal stromal Cells for future use in regenerative medicine

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Study on Viability and Chondrogenic Differentiation of Cryopreserved Adipose Tissue-Derived Mesenchymal stromal Cells for future use in regenerative medicine

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Título: Study on Viability and Chondrogenic Differentiation of Cryopreserved Adipose Tissue-Derived Mesenchymal stromal Cells for future use in regenerative medicine
Autor: González-Fernández, María Luisa;Pérez-Castrillo, S.;Ordás-Fernández, P.;López-González, M.E.;Colaço, B.;Villar-Suárez, V.
Facultad/Centro: Instituto Universitario de Biomedicina (IBIOMED)
Area de conocimiento: Otros
Resumen: Adipose-derived mesenchymal stromal cells are promising as a regenerative therapy tool for defective tissues in mesenchymal lineage, including fat, bone, cartilage, and blood vessels. In potential future clinical applications, adipose-derived stem cell cryopreservation is an essential fundamental technology. The aim of this study is to define an adequate protocol for the cryopreservation of adipose-derived mesenchymal stromal cells, by comparing various protocols so as to determine the effects of cryopreservation on viability and chondrogenic differentiation potential of adipose-derived stem cells upon freeze-thawing of AT-MSCs colonies cryopreserved with standard and modified protocols, using flow cytometry and confocal microscopy. The study concludes that adipose-derived mesenchymal stromal cells could be long-term cryopreserved without any loss of their proliferative or differentiation potential
Descripción física: P. 256–263
Revisión por pares: SI
Editor: Elsevier
Datos: Cryobiology (2015), n. 71
URI: http://hdl.handle.net/10612/6449
Fecha: 2015-07-23
Tipo: info:eu-repo/semantics/article
Materia: Anatomía
Veterinaria
Palabras clave: Cryopreservation
Crioperservación
Chondrogenic differentiation
Medicina regenerativa
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