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    Citas

    Título
    Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments
    Autor
    Domínguez Rebolledo, Álvaro Efrén
    Fernández Santos, María Rocío
    Bisbal Vigo, Alfonso
    Ros Santaella, José Luis
    Ramón Fernández, Manuel
    Carmona Delgado, Manuel
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Garde López-Brea, Julián
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Reproduction, Fertility and Development, 2010, vol. 22, n. 5
    Editor
    CSIRO
    Fecha
    2010-04-15
    Abstract
    Antioxidants could improve sperm media, extending the viability of spermatozoa and protecting their DNA. The protective ability of lipoic acid, melatonin, Trolox and crocin was tested on red deer spermatozoa incubated at 37°C. Cryopreserved spermatozoa were thawed and incubated with 1 mM or 0.1 mM of each antioxidant, with or without oxidative stress (100 μM Fe2+). Motility (CASA), viability, mitochondrial membrane potential and acrosomal status were assessed. Lipoperoxidation (malondialdehyde production), intracellular reactive oxygen species (ROS) and DNA status (TUNEL) were checked at 4 h. Incubation alone increased ROS and decreased motility. Oxidative stress intensified these effects, increasing lipoperoxidation and DNA damage. Lipoic acid had little protective effect, whereas 1 mM melatonin showed limited protection. Trolox lowered ROS and lipoperoxidation both in oxidised and non-oxidised samples. In oxidised samples, Trolox prevented DNA and acrosomal damage, and ameliorated motility. Crocin at 1 mM showed similar results to Trolox, but noticeably stimulated motility and had no effect on lipoperoxidation. In a second experiment, a broader range of crocin and melatonin concentrations were tested, confirming the effects of crocin (positive effects noticeable at 0.5–0.75 mM), but showing an increase in lipoperoxidation at 2 mM. Melatonin was increasingly effective at 2.5 and 5 mM (ROS, lipoperoxidation and DNA status). Crocin seems a promising new antioxidant, but its particular effects on sperm physiology must be further studied, especially the consequences of motility stimulation and confirming its effect on lipoperoxidation. Melatonin might be useful at relatively high concentrations, compared to Trolox.
    Materia
    Veterinaria
    Palabras clave
    Crocin
    Lipoic acid
    Melatonin
    Trolox
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10222
    Versión del editor
    http://www.publish.csiro.au/rd/rd09197
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