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    Título
    Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) spermatozoa obtained by electroejaculation
    Autor
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Martínez, Félix
    Álvarez García, MercedesAutoridad BuleriaORCID
    Maroto Morales, Alejandro
    García Álvarez, Olga
    Soler, Ana J.
    Garde López-Brea, Julián
    Paz Cabello, Paulino deAutoridad BuleriaORCID
    Anel Rodríguez, LuisAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Theriogenology, 2009, vol. 71, n. 4
    Editor
    Elsevier
    Fecha
    2009-03-01
    Abstract
    We tested extenders and freezing protocols for Iberian red deer semen. Samples were obtained by electroejaculation (10 stags), and analyzed for motility (CASA), viability (propidium ioide), acrosomal (PNA-FITC) and mitochondrial status (JC-1). Samples were diluted in extender, cooled and adjusted for glycerol (extender with higher glycerol concentration), brought to mL−1 and frozen. Four experiments were carried out, repeating sperm analysis after thawing to compare treatments. In a first experiment, seven samples were frozen using Triladyl® (20% egg yolk) and UL extender (Tes–Tris–fructose, 15% egg yolk, 4% glycerol). Triladyl® yielded higher motility after thawing. In a second trial, 17 samples were frozen using Triladyl®, Andromed®, Bioxcell®, and UL with 8% LDL (low-density lipoproteins). Triladyl® and Andromed® performed better than Bioxcell® on motility, and than UL-LDL on viability and acrosomal status. In a third experiment, the performance of freezing the sperm-rich ejaculate fraction versus the whole ejaculate was tested on nine samples. The sperm-rich ejaculate fraction not only rendered more motile and viable spermatozoa but also showed higher freezability (higher motile spermatozoa recovery). In a fourth experiment, we tried three modifications of the freezing protocol, for improving the freezability of low concentration samples: prior removal of seminal plasma; replacing extender (second fraction) for pure glycerol to reduce dilution; and performing only the dilution, not the second dilution. No differences were found, although only three samples could be used. Both Triladyl® and Andromed® were deemed appropriate for freezing Iberian red deer semen, and the rich fraction should be selected for freezing.
    Materia
    Veterinaria
    Palabras clave
    Sperm cryopreservation
    Electroejaculation
    Extenders
    Red deer
    Semen
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10288
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0093691X08006857#!
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