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    Citas

    Título
    Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation
    Autor
    Fernández Santos, María Rocío
    Domínguez Rebolledo, Álvaro Efrén
    Esteso, Milagros
    Garde López-Brea, Julián
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Reproduction in Domestic Animals, 2009, vol. 44, n. 2
    Editor
    John Wiley & Sons
    Fecha
    2009-04
    Abstract
    We have approached the problem of refrigerated storage of epididymal sperm samples from red deer by comparing three options: storing the genital (testicles within the scrotum), diluting the semen in extender or diluting the semen in extender supplemented with an anti‐oxidant. Twenty‐nine pairs of testes were collected. Spermatozoa from one of each of the pairs were immediately recovered, and diluted to 400 × 106 sperm/ml in Tris‐citrate‐fructose with 20% egg yolk. Control group was stored as such, and Anti‐oxidant group was supplemented with 0.8 mm vitamin C. The remaining epididymides and the diluted samples were stored at 5°C and spermatozoa were analysed at 0, 24, 96 and 192 h for: motility [computer‐assisted semen analysis (CASA)], acrosomal integrity, sperm viability (eosine/nigrosine staining), normal tails and chromatin status [sperm chromatin structure assay (SCSA)]. In general, seminal quality decreased with storage time. Vitamin C supported progressive motility better at 24 h (median 42% vs 23% Control and 15% epididymis), reduced the incidence of tail abnormalities and protected chromatin. Storing the semen in the epididymis slowed down motility loss, but slightly increased the occurrence of tail abnormalities and viability was lower at 192 h. However, regarding chromatin status, sperm stored in the epididymis was protected similarly to those diluted in the medium supplemented with vitamin C. Although the differences between the three groups were small, there were some advantages in supplementing the extender with vitamin C. Besides, refrigerating the epididymis may be a good option when immediate processing is not available.
    Materia
    Veterinaria
    Palabras clave
    Esperma
    Refrigeración
    Ciervo rojo
    Cromatina
    CASA
    Epidídimo
    Vitamina C
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10321
    Versión del editor
    https://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0531.2007.01032.x
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    28 Refrigerated Storage of Red Deer Epididymal Spermatozoa ReprodDomestAnim_044_0212_2009.pdf
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