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    Título
    Rainbow trout surviving infections of viral haemorrhagic septicemia virus (vhsv) show lasting antibodies to recombinant g protein fragments
    Autor
    Gómez-Casado, E.
    Fregeneda Grandes, Juan MiguelAutoridad BuleriaORCID
    Olesen, Niels Jørgen
    Lorenzen, Niels
    Estepa Pérez, Amparo
    Coll Morales, Julio
    Encinas, Paloma
    Facultad/Centro
    Facultad de Veterinaria
    Área de conocimiento
    Sanidad Animal
    Datos de la obra
    Fish and Shellfish Immunology, 2011, vol. 30, n. 3
    Editor
    Elsevier
    Fecha
    2011-03-15
    Abstract
    Rainbow trout antibodies (Abs) binding to recombinant fragments (frgs) derived from the protein G of the viral haemorrhagic septicemia virus (VHSV)-07.71 strain, could be detected by ELISA (frg-ELISA) in sera from trout surviving laboratory-controlled infections. Abs were detected not only by using sera from trout infected with the homologous VHSV isolate but also with the VHSV-DK-201433 heterologous isolate, which had 13 amino acid changes. Sera from healthy trout and/or from trout surviving infectious haematopoietic necrosis virus (IHNV) infection, were used to calculate cut-off absorbances to differentiate negative from positive sera. Specific anti-VHSV Abs could then be detected by using any of the following frgs: frg11 (56-110), frg15 (65-250), frg16 (252-450) or G21-465. While high correlations were found among the ELISA values obtained with the different frgs, no correlations between any frg- ELISA and complement-dependent 50 % plaque neutralization test (PNT) titres could be demonstrated. Between 4 to 10 weeks after VHSV-infection, more trout sera were detected as positives by using heterologous frg-ELISA rather than homologous PNT. Furthermore, the percentage of positive sera detected by frg11-ELISA increased with time after infection to reach 100 %, while those detected by complement-dependent PNT decreased to 29.4 %, thus confirming that the lack of neutralising Abs does not mean the lack of any anti-VHSV Abs in survivor trout sera. Preliminary results with sera from field samples suggest that further refinements of the frg-ELISA could allow detection of anti-VHSV trout Abs in natural outbreaks caused by different heterologous VHSV isolates.The homologous frg-ELISA method could be useful to follow G immunization attempts during vaccine development and/or to best understand the fish Ab response during VHSV infections. The viral frgs approach might also be used with other fish species and/or viruses.
    Materia
    Inmunología
    Sanidad animal
    Veterinaria
    Zoología
    Palabras clave
    Truchas
    Inmunoglobulinas
    Serología
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10571
    Versión del editor
    https://doi.org/10.1016/j.fsi.2011.01.021
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