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    Citas

    Título
    Response of Thawed Epidi dymal Red Deer Spermatozoa to Increasing Concentrations of Hydrogen Peroxide, and Importance of Individual Male Variability
    Autor
    Domínguez Rebolledo, Álvaro Efrén
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Bisbal Vigo, Alfonso
    Ros Santaella, José Luis
    García Álvarez, Olga
    Maroto Morales, Alejandro
    Soler, Ana J.
    Garde López-Brea, Julián
    Fernández Santos, María Rocío
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Reproduction in Domestic Animals, 2011, vol. 46, n. 3
    Editor
    John Wiley & Sons
    Fecha
    2011-06
    Abstract
    Oxidative stress represents a challenge during sperm manipulation. We have tested the effect of increasing hydrogen peroxide (H2O2) levels on red deer spermatozoa after cryopreservation, and the role of male‐to‐male variation in that response. In a first experiment, eight thawed samples were submitted to 0, 25, 50, 100 and 200 μm H2O2 for 2 h at 37°C. Intracellular reactive oxygen species (H2DCFDA‐CM) increased with H2O2 concentration, but we only detected a decrease in sperm function (motility by CASA and chromatin damage by sperm chromatin structure assay) with 200 μm. Lipoperoxidation assessed by the thiobarbituric acid reactive substance (TBARS) method increased slightly with 50 μm H2O2 and above. In a second experiment, samples from seven males were submitted to 0 and 200 μm H2O2 for 2 h, triplicating the experiment within each male. Males differed at thawing and regarding their response to incubation and H2O2 presence. We found that the kinematic parameters reflected male‐to‐male variability, whereas the response of the different males was similar for lipid peroxidation and viability. A multiparametric analysis showed that males grouped differently if samples were assessed after thawing, after incubation without H2O2 or after incubation with H2O2. Red deer spermatozoa are relatively resilient to H2O2 after thawing, but it seems to be a great male‐to‐male variability regarding the response to oxidative stress. The acknowledgement of this individual variability might improve the development of optimized sperm work protocols.
    Materia
    Veterinaria
    Palabras clave
    Ciervo rojo
    Esperma
    Agua
    Estrés oxidativo
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10612
    Versión del editor
    https://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0531.2010.01677.x
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