• español
  • English
  • français
  • Deutsch
  • português (Brasil)
  • italiano
  • Contact Us
  • Send Feedback
  • Enlaces y accesos
    • Derechos de autor
    • Políticas
    • Guía de autoarchivo
    • FAQ y ayuda
    • La ULE y el Acceso Abierto
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Repositorio
    Institucional
    Abierto

    Browse

    All of BULERIACommunities and CollectionsAuthorsDirectoresTitlesSubjectsFacultad/CentroÁrea de conocimientoFecha de creación/publicaciónTitulaciónThis CollectionAuthorsDirectoresTitlesSubjectsFacultad/CentroÁrea de conocimientoFecha de creación/publicaciónTitulación

    My Account

    Login

    Statistics

    View Usage Statistics

    Otros enlaces

    View Item 
    •   BULERIA Home
    • Scientific Production
    • Artículos de revista (post-print)
    • View Item
    •   BULERIA Home
    • Scientific Production
    • Artículos de revista (post-print)
    • View Item

    Compartir

    Export

    RISMendeleyRefworksZotero
    • edm
    • marc
    • xoai
    • qdc
    • ore
    • ese
    • dim
    • uketd_dc
    • oai_dc
    • etdms
    • oai_openaire
    • rdf
    • mods
    • mets
    • didl
    • premis

    Citas

    Academic Search
    Título
    Effect of Several Antioxidants on Thawed Ram Spermatozoa Submitted to 37°C up to Four Hours
    Autor
    Mata Campuzano, María
    Álvarez Rodríguez, Manuel
    Álvarez García, Mercedes
    Anel Rodríguez, LuisAutoridad Buleria
    Paz Cabello, Paulino de
    Garde López-Brea, Julián
    Martínez Pastor, FelipeAutoridad Buleria
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Reproduction in Domestic Animals, 2012, vol. 47, n. 6
    Editor
    John Wiley & Sons
    Fecha
    2012-12
    Descripción física
    P. 907-914
    Abstract
    Thawed ram spermatozoa were incubated at 37°C in the presence of dehydroascorbic acid (DHA), TEMPOL (TPL), N‐acetyl‐cysteine (NAC) and rutin (RUT), at 0.1 and 1 mm, in order to test their effects on sperm physiology. Cryopreserved spermatozoa from four rams were thawed, pooled, washed and incubated in TALP‐Hepes with 1 mm or 0.1 mm of each antioxidant, performing a replicate with induced oxidative stress (Fe2+/ascorbate). Motility (CASA), viability and mitochondrial membrane potential (flow cytometry) were analysed at 2 and 4 h. Lipoperoxidation (MDA production), intracellular reactive oxygen species (ROS) and DNA status (TUNEL) were analysed at 4 h. Antioxidants, except DHA 0.1 mm, decreased motility and kinematic parameters, but had little effect on viability or mitochondrial activity. Except 1 mm DHA, the antioxidants reduced ROS at 4 h. Moreover, NAC 1 mm, rutin and TEMPOL reduced ROS and DNA damage in the presence of oxidative stress. N‐acetyl‐cysteine, rutin 1 mm and TEMPOL reduced lipoperoxidation in the presence of oxidative stress. However, DHA did not affect lipoperoxidation. At 1 mm, DHA increased DNA damage in the absence of oxidative stress. Dehydroascorbic acid effects could arise from spermatozoa having a low capacity for reducing it to ascorbic acid, and it may be tested in the presence of other antioxidants or reducing power. Future research should focus in testing whether the inhibition of motility observed for NAC, rutin and TEMPOL is reversible. These antioxidants might be useful at lower temperatures (refrigerated storage or cryopreservation) when their protective effects could be advantageous.
    Materia
    Veterinaria
    Palabras clave
    Antioxidantes
    Estrés oxidativo
    Ganado ovino
    Esperma
    Idioma
    eng
    Tipo documental
    info:eu-repo/semantics/article
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10656
    Versión del editor
    https://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2012.01990.x
    Collections
    • Artículos de revista (post-print) [1546]
    Show full item record
    Files in this item
    Nombre:
    54 Effect of Several Antioxidants ReprodDomestAnim_047_0907_2012.pdf
    Tamaño:
    217.3Kb
    Formato:
    Adobe PDF
    Thumbnail
    FilesOpen