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    Título
    Spermatozoa recovery and post-thawing quality of brown bear ejaculates is affected for centrifugation regimes
    Autor
    Nicolás, M.
    Álvarez García, Mercedes
    Anel Rodríguez, Enrique
    Martínez, Félix
    Barragán, Santiago
    Martínez Pastor, FelipeAutoridad Buleria
    Paz Cabello, Paulino de
    Anel Rodríguez, LuisAutoridad Buleria
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    European Journal of Wildlife Research, 2012, vol. 58, n. 1
    Editor
    Springer
    Fecha
    2012-02
    Descripción física
    P. 77–84
    Abstract
    Sperm cryopreservation protocols for brown bear (Ursus arctos) require the centrifugation of semen samples to increase sperm concentration and to clean urine in contaminated samples. We evaluated the effect of centrifugation regimes (time and relative centrifugal force—RCF) on the quantity of sperm recovered and the quality of post-thawed sperm. Thirteen brown bears were electroejaculated. The ejaculates were diluted 1:1 in Tris–citric acid–glucose (TCG) extender and centrifuged with different RCF/time combinations: 600×g, 1,200×g and 2,400×g, for 3, 6 or 12 min. After centrifugation, spermatozoa were diluted in TES–Tris–fructose extender with egg yolk and glycerol (final glycerol concentration of 8%) and frozen in 0.25-mL straws. In the post-thawed semen, motility was assessed by CASA, and acrosomal status (PNA-FITC), viability (SYBR-14 with propidium iodide) and chromatin status (SCSA) were determined by flow cytometry. The longest centrifugation time (12 min) significantly decreased some motility parameters. Sperm recovery significantly decreased in brown bear at 600×g. Our results suggest that brown bear spermatozoa are more sensitive to long centrifugation times than to high RCF. Centrifugation regimes showed no effects on the post-thawing chromatin status. We recommend preparing the brown bear semen for freezing by centrifugation 1,200×g or 2,400×g for 6 min, after electroejaculation and dilution 1:1 in TCG extender, since these procedures increase the spermatozoa recovery without harmful effects on the post-thawed quality of brown bear spermatozoa.
    Materia
    Veterinaria
    Palabras clave
    Centrifugation
    Cryopreservation
    Brown bear
    Spermatozoa
    Idioma
    eng
    Tipo documental
    info:eu-repo/semantics/article
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10694
    Versión del editor
    https://link.springer.com/article/10.1007/s10344-011-0544-8
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    • Artículos de revista (post-print) [1543]
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