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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorNicolás, M.
dc.contributor.authorÁlvarez García, Mercedes
dc.contributor.authorAnel Rodríguez, Enrique
dc.contributor.authorMartínez, Félix
dc.contributor.authorBarragán Santos, Santiago
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorPaz Cabello, Paulino de
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.otherBiologia Celulares_ES
dc.date2012-02
dc.date.accessioned2019-05-12T23:10:45Z
dc.date.available2019-05-12T23:10:45Z
dc.date.issued2019-05-13
dc.identifier.citationEuropean Journal of Wildlife Research, 2012, vol. 58, n. 1es_ES
dc.identifier.otherhttps://link.springer.com/article/10.1007/s10344-011-0544-8es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10694
dc.descriptionP. 77–84es_ES
dc.description.abstractSperm cryopreservation protocols for brown bear (Ursus arctos) require the centrifugation of semen samples to increase sperm concentration and to clean urine in contaminated samples. We evaluated the effect of centrifugation regimes (time and relative centrifugal force—RCF) on the quantity of sperm recovered and the quality of post-thawed sperm. Thirteen brown bears were electroejaculated. The ejaculates were diluted 1:1 in Tris–citric acid–glucose (TCG) extender and centrifuged with different RCF/time combinations: 600×g, 1,200×g and 2,400×g, for 3, 6 or 12 min. After centrifugation, spermatozoa were diluted in TES–Tris–fructose extender with egg yolk and glycerol (final glycerol concentration of 8%) and frozen in 0.25-mL straws. In the post-thawed semen, motility was assessed by CASA, and acrosomal status (PNA-FITC), viability (SYBR-14 with propidium iodide) and chromatin status (SCSA) were determined by flow cytometry. The longest centrifugation time (12 min) significantly decreased some motility parameters. Sperm recovery significantly decreased in brown bear at 600×g. Our results suggest that brown bear spermatozoa are more sensitive to long centrifugation times than to high RCF. Centrifugation regimes showed no effects on the post-thawing chromatin status. We recommend preparing the brown bear semen for freezing by centrifugation 1,200×g or 2,400×g for 6 min, after electroejaculation and dilution 1:1 in TCG extender, since these procedures increase the spermatozoa recovery without harmful effects on the post-thawed quality of brown bear spermatozoa.es_ES
dc.languageenges_ES
dc.publisherSpringeres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherCentrifugationes_ES
dc.subject.otherCryopreservationes_ES
dc.subject.otherBrown beares_ES
dc.subject.otherSpermatozoaes_ES
dc.titleSpermatozoa recovery and post-thawing quality of brown bear ejaculates is affected for centrifugation regimeses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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