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    Título
    Outlining adequate protocols for Lidia bull epididymal storage and sperm cryopreservation: use of glycerol, dimethylformamide and N-acetylcysteine
    Autor
    Matilla Pinto, Elvira
    González Fernández, Lauro
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Hernández Rollán, Nuria
    Tobajas Vega, Carolina
    Calle Guisado, Violeta
    Mijares Gordún, José
    Sánchez Margallo, Francisco Miguel
    Álvarez Miguel, Ignacio Santiago
    Macías García, Beatriz
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Spanish Journal of Agricultural Research, 2017, vol. 15, n. 3
    Editor
    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    Fecha
    2017
    Abstract
    The Lidia bovine breed is an important hallmark of the Spanish cattle industry. Bulls are selected based upon aggressiveness and epididymal sperm cryopreservation is the way to obtain and store their genetics. There are not specifically designed protocols yet to perform Lidia bull sperm cryopreservation. The present study aimed to determine if a tris-fructose-citrate-egg yolk (20% v/v; TFY) extender supplemented with 7% glycerol (TFY1) or 3.5% glycerol plus 3.5% dimethylformamide (DMF; TFY2) are suitable media for cryopreservation of epididymal Lidia bull sperm. Moreover, the effect of N-acetylcysteine (NAC), a potent antioxidant, was evaluated. The epididymis were stored at 4°C for 24, 48, 72 or 96 h, and both freezing media were tested as such or supplemented with 1 or 2.5 mM of NAC. Our data demonstrated that post-thaw viability was well maintained (TFY1: 50.8% ± 1.9 at 24 h and 52.4% ± 0.8 at 96 h and TFY2: 52.6% ± 1.6 at 24 h and 56.1% ± 1.8 at 96 h; mean % ± SEM; p>0.05) as also were total and progressive sperm motility, high mitochondrial membrane potential, ROS production, DNA status and acrosomal intactness of Lidia bull sperm up to 96 h of epididymal storage, all extender variations being similar (p>0.05). In conclusion, the use of TFY medium supplemented either with 7% glycerol alone or the combination of 3.5% glycerol and 3.5% DMF were equally safe choices for epididymal Lidia bull sperm cryopreservation, and NAC addition did not significantly improve sperm post-thaw quality.
    Materia
    Veterinaria
    Palabras clave
    Fighting bull
    Epididymal sperm
    Sperm freezing extenders
    Antioxidants
    Cooling
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10719
    Versión del editor
    http://revistas.inia.es/index.php/sjar/article/view/11463
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