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    Título
    Removal of bacteria from boar semen using a low-density colloid
    Autor
    Morrell, Jane Margaret
    Núñez González, A.
    Crespo Félez, I.
    Martínez Martínez, SoniaAutoridad BuleriaORCID
    Martínez Alborcia, María J.
    Fernández Alegre, Estela
    Domínguez Fernández de Tejerina, Juan CarlosAutoridad BuleriaORCID
    Gutiérrez Martín, César BernardoAutoridad BuleriaORCID
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Theriogenology, 2019, vol. 126
    Editor
    Elsevier
    Fecha
    2019-03-01
    Abstract
    Antibiotics are added to semen extenders when preparing commercial semen doses for artificial insemination according to national and international guidelines. However, this addition of antibiotics represents non-therapeutic usage and could be contributing to the development of antibiotic resistance. Colloid centrifugation was shown to reduce the load of bacteria present in boar semen and was capable of removing all bacteria if performed directly after semen collection, albeit with some loss of spermatozoa. The present experiment was conducted with a low density colloid to investigate whether it was possible to separate all of the spermatozoa from seminal plasma i.e. without selection for robust spermatozoa, or whether this would have a detrimental effect on sperm quality. Ejaculates from nine boars were extended in Beltsville Thawing Solution without antibiotics and were transported to the laboratory for Single Layer Centrifugation (SLC) on modified Porcicoll i.e. at a low density (S). A further modification was that a sterile inner tube was included inside some of the 50 mL centrifuge tubes to facilitate harvesting of the sperm pellet (M). Aliquots of all samples (control, S and M) were cultured for bacterial quantification and identification using standard microbiological methods. Sperm quality was evaluated daily. Three of the C and M samples and five of the S samples did not contain any bacteria. Mean bacterial counts for the remaining samples (colony forming units/mL) were as follows: C 259 ± 216; S 30 ± 22; M 33 ± 15 (P < 0.01). Citrobacter spp., Staphylococcus simulans, Klebsiella variicola, Escherichia coli, Myroides odoratimimus, Proteus spp. and Enterococcus faecalis were identified in the control samples. There were marginal differences in sperm quality among treatments, with sperm velocity and linearity being higher in S and M samples than in C at all time points. However, sperm viability, capacitation and acrosome status were marginally better in controls than in S or M on day 0, but these differences disappeared during storage. Conclusions: centrifugation through a low density colloid can remove or reduce bacterial contamination in boar ejaculates without using antibiotics. Furthermore, it is possible to collect boar ejaculates without bacterial contamination by paying strict attention to hygiene.
    Materia
    Veterinaria
    Palabras clave
    Jabalíes
    Semen
    Centrifugación
    Coloides
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10808
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0093691X18308331#!
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