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Título
Modulation of multiple gene clusters’ expression by the PAS-LuxR transcriptional regulator PteF
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Antibiotics
Número de la revista
8
Cita Bibliográfica
Vicente, C. M., Payero, T. D., Rodríguez-García, A., Barreales, E. G., Pedro, A. d., Santos-Beneit, F., & Aparicio, J. F. (2022). Modulation of multiple gene clusters’ expression by the PAS-LuxR transcriptional regulator PteF. Antibiotics, 11(8), Article e994. https://doi.org/10.3390/ANTIBIOTICS11080994
Editorial
MDPI
Fecha
2022-07-24
Resumen
[EN] PAS-LuxR transcriptional regulators are conserved proteins governing polyene antifungal biosynthesis. PteF is the regulator of filipin biosynthesis from Streptomyces avermitilis. Its mutation drastically abates filipin, but also oligomycin production, a macrolide ATP-synthase inhibitor, and delays sporulation; thus, it has been considered a transcriptional activator. Transcriptomic analyses were performed in S. avermitilis ΔpteF and its parental strain. Both strains were grown in a YEME medium without sucrose, and the samples were taken at exponential and stationary growth phases. A total of 257 genes showed an altered expression in the mutant, most of them at the exponential growth phase. Surprisingly, despite PteF being considered an activator, most of the genes affected showed overexpression, thereby suggesting a negative modulation. The affected genes were related to various metabolic processes, including genetic information processing; DNA, energy, carbohydrate, and lipid metabolism; morphological differentiation; and transcriptional regulation, among others, but were particularly related to secondary metabolite biosynthesis. Notably, 10 secondary metabolite gene clusters out of the 38 encoded by the genome showed altered expression profiles in the mutant, suggesting a regulatory role for PteF that is wider than expected. The transcriptomic results were validated by quantitative reverse-transcription polymerase chain reaction. These findings provide important clues to understanding the intertwined regulatory machinery that modulates antibiotic biosynthesis in Streptomyces
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MINECO/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/BIO2013-42983-P/ES/REGULADORES DE LA PRODUCCION DE MACROLIDOS ANTIFUNGICOS PARA EL DESCUBRIMIENTO DE NUEVOS FARMACOS
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DOI
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