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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Cánovas, Sebastian | |
dc.contributor.author | Ivanova, Elena | |
dc.contributor.author | Romar, Raquel | |
dc.contributor.author | García Martínez, Soledad | |
dc.contributor.author | Soriano Úbeda, Cristina de las Mercedes | |
dc.contributor.author | García Vázquez, Francisco Alberto | |
dc.contributor.author | Saadeh, Heba | |
dc.contributor.author | Andrews, Simon | |
dc.contributor.author | Kelsey, Gavin | |
dc.contributor.author | Coy, Pilar | |
dc.contributor.other | Medicina y Cirugia Animal | es_ES |
dc.date | 2017 | |
dc.date.accessioned | 2024-03-05T12:55:28Z | |
dc.date.available | 2024-03-05T12:55:28Z | |
dc.identifier.citation | Canovas, S., Ivanova, E., Romar, R., García-Martínez, S., Soriano-Úbeda, C., García-Vázquez, F. A., Saadeh, H., Andrews, S., Kelsey, G., & Coy, P. (2017). DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids. eLife, 6. https://doi.org/10.7554/ELIFE.23670 | es_ES |
dc.identifier.other | https://elifesciences.org/articles/23670 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/18601 | |
dc.description.abstract | [EN]The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first wholegenome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than C-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showed that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts. Genes involved in reprogramming, imprinting and development were affected by culture, with fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and NNAT. | es_ES |
dc.language | eng | es_ES |
dc.publisher | eLife Sciences Publications | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Reproducción | es_ES |
dc.subject.other | Embriones | es_ES |
dc.subject.other | Fecundación in vitro | es_ES |
dc.subject.other | Cerdos | es_ES |
dc.title | DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.7554/eLife.23670 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/ Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2015-66341-R/ES/OBTENCION DE ANIMALES SANOS MEDIANTE TECNICAS DE REPRODUCCION ASISTIDA BASADAS EN CONDICIONES FISIOLOGICAS | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ MECD/Programa Estatal de Promoción del Talento y su Empleabilidad/ PRX14/00348/ | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 2050-084X | |
dc.journal.title | eLife | es_ES |
dc.volume.number | 6 | es_ES |
dc.page.initial | e23670 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 3104 Producción Animal | es_ES |
dc.subject.unesco | 2411.16 Fisiología de la Reproducción | es_ES |
dc.description.project | The authors thank CEFU, SA and El Pozo, SA for providing the biological material; Juan Antonio Carvajal and Soledad Rodriguez for collecting the oviducts, uteri and ovaries at the slaughterhouse; Carmen Mata´ s for technical support with IVF and Kristina Tabbada for sequencing RNA-Seq and BS-seq libraries. Funding: Work in GK’s laboratory was supported by the UK Biotechnology and Biological Sciences Research Council and Medical Research Council. Work in PC’s laboratory was supported by grants AGL2012–40180 C03-01 and AGL2015–66341-R from the Ministry of Economy and Competitiveness (Spain), and 20040/GERM/16 from Fundacio´ n Se´ neca. PC stay at The Babraham Institute was funded by a mobility grant of the Spanish Ministry of Education, Culture and Sports (PRX14/00348) | es_ES |
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