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Título
DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
eLife
Cita Bibliográfica
Canovas, S., Ivanova, E., Romar, R., García-Martínez, S., Soriano-Úbeda, C., García-Vázquez, F. A., Saadeh, H., Andrews, S., Kelsey, G., & Coy, P. (2017). DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids. eLife, 6. https://doi.org/10.7554/ELIFE.23670
Editorial
eLife Sciences Publications
Fecha
2017
Resumen
[EN]The number of children born since the origin of Assisted Reproductive Technologies
(ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been
reported among ART-conceived infants, suggesting an epigenetic cost. We report the first wholegenome DNA methylation datasets from single pig blastocysts showing differences between in vivo
and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the
presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than
C-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showed
that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts.
Genes involved in reprogramming, imprinting and development were affected by culture, with
fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in
C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and
NNAT.
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MINECO/ Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2015-66341-R/ES/OBTENCION DE ANIMALES SANOS MEDIANTE TECNICAS DE REPRODUCCION ASISTIDA BASADAS EN CONDICIONES FISIOLOGICAS
- info:eu-repo/grantAgreement/ MECD/Programa Estatal de Promoción del Talento y su Empleabilidad/ PRX14/00348/
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DOI
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